A method of isolating and purifying ()-Ambrox from a reaction mixture comprising ()-Ambrox and one or more of the compounds (II), (III) and (IV)

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Provided is a method for producing ambrein, comprising reacting tetraprenyl--curcumene cyclase with 3-deoxyachilleol A to obtain ambrein.

The disclosure relates to sesquiterpene homologues, their use as fragrance and a method of their production.

A solid form of ()-Ambrox formed by a bioconversion process.

Improved methods of making amberketal and amberketal homologues and compositions comprising same, improved squalene-hopene cyclase (SHC) enzymes to be used in said methods, nucleic acid constructs and vectors encoding said enzymes, and host cells expressing said enzymes.

A method for purifying a crude flavor or perfumery or cosmetic ingredient or intermediate, for example crude ()-Ambrox, comprising a number of washing steps, the products of said method, and uses of said product.

Squalene Hopene Cyclase (SHC) enzymes and variants thereof and their uses for making ()-Ambrox from homofarnesol and Ambra oxide from bishomofarnesol.

Squalene Hopene Cyclase (SHC) enzymes and variants thereof and their uses for making ()-Ambrox from homofarnesol and Ambra oxide from bishomofarnesol.

Variants of squalene hopene cyclase (SHC) are provided for enzymatically converting homofarnesoic acid to sclareolide, which can be non-enzymatically converted to ambrox.

The present invention relates to genetically-modified oleaginous yeasts for producing non-native sterols at commercially useful levels, especially for example in providing sterols individually or as a mixture in an artificial dietary composition for honeybees or other insects or animals. For this purpose, an oleaginous yeast, e.g. Yarrowia lipolytica, may be employed wherein the yeast has reduced production of ergosterol compared with a wild-type oleaginous yeast or is incapable of producing ergosterol and is provided with a sterol surrogate to aid growth. From such yeast, however, other yeast may be engineered which retain useful sterol production without need for a sterol surrogate, e.g. production of sterol mixtures in which 24-methylenecholesterol or campesterol is the dominant sterol.