The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for creating novel herbicide resistant plants by base editing techniques and a method for screening endogenous gene mutation sites capable of conferring herbicide resistance in plants. The invention also relates to the use of the identified endogenous gene mutantation sites in crop breeding.

The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

The present invention provides for a genetically modified yeast cell comprising at least six or more of the following modifications: increased expression of Mus musculus fatty acid reductase, acetyl-CoA carboxylase, fatty acid synthase 1, fatty acid synthase 2, a mutant of the bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression, and/or a desaturase encoded by OLE1, and reduced expression of DGA1, HFD1, ADH6, and/or GDH1. The present invention provides a method for constructing the genetically modified yeast cell, and a method for producing a fatty alcohol from the genetically modified yeast cell.

The herbicide-tolerant rice cultivar designated “PVL01” and its hybrids and derivatives are disclosed. The present invention provides a method for producing rice, wherein said rice is tolerant to a quizalofop herbicide or tolerant to a tepraloxdyim herbicide; wherein said rice belongs to any of (a) variety “PVL01” or (b) a hybrid, derivative, or progeny of “PVL01”. Further provided are methods for producing a hybrid or a new variety by crossing the rice variety “PVL01” with another rice line, one or more times. In another embodiment, this invention allows for single-gene converted plants of “PVL01”. In another embodiment, this invention provides regenerable cells for use in tissue culture of rice plant “PVL01”.

The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for creating novel herbicide resistant plants by base editing techniques and a method for screening endogenous gene mutation sites capable of conferring herbicide resistance in plants. The invention also relates to the use of the identified endogenous gene mutantation sites in crop breeding.

The present invention provides the peptide nucleic acid derivative which targets 5′ splice site of the human ACC2 pre-mRNA “exon 12”. The peptide nucleic acid derivatives in the present invention strongly induce splice variants of the human ACC2 mRNA in cell and are very useful to treat conditions or disorders of skin aging associated with the human ACC2 protein.

The herbicide-tolerant rice cultivar designated ‘PVL02’ and its hybrids and derivatives are disclosed.

This document relates to using bidirectional, multi-enzymatic scaffolds to biosynthesize cannabinoids in recombinant hosts.

A mutant acetyl-CoA carboxylase (ACC) protein, a nucleic acid encoding the mutant ACC protein, and use thereof are provided. Specifically, compared with a parent ACC protein, the mutant ACC protein has mutations at amino acids corresponding to amino acid 1,879 and/or amino acid 2,186 of SEQ ID NO: 1. An ACC-mutated plant shows high herbicide resistance, and thus the present disclosure has very promising application prospects in the cultivation of an herbicide-resistant plant.

The present invention relates to the field of plant genetic engineering. In particular, the present invention relates to acetolactate synthase (ALS) mutants, protoporphyrinogen oxidase (PPO) mutants, acetyl-CoA carboxylase (ACCase) mutants and/or p-hydroxyphenylpyruvate dioxidase (HPPD) mutants capable of conferring herbicide resistance in plants, especially in wheat and/or rice plants, and methods for production herbicide-resistant plants, especially wheat and/or rice plants comprising said acetolactate synthase (ALS) mutants and/or protoporphyrinogen oxidase (PPO) mutants and/or acetyl-CoA carboxylase (ACCase) mutants and/or p-hydroxyphenylpyruvate dioxidase (HPPD) mutants.