A method for detecting fluid injection in a patient, the method including the steps of providing a fluid storage tank; providing fluid for use in machinery and adding said fluid to the fluid storage tank; and providing a fluorescent dye and adding the fluorescent dye to the fluid such that the fluid fluoresces in the presence of ultraviolet light.

A method and device for determining the depth and fluorophore concentration of a fluorophore concentration below the surface of an optically absorbing and scattering medium suitable for use in fluorescence-based surgical guidance such as in tumor resection is described. Long-wavelength stimulus light us used to obtain deep tissue penetration. Recovery of depth is performed by fitting measured modulation amplitudes for each spatial frequency to precomputed modulation amplitudes in a table of modulation amplitudes indexed by optical parameters and depth.

A method for determining a glomerular filtration rate (GFR) in a patient includes administering to said patient a compound of Formula I and transdermally measuring spectral energy emitted by the compound of Formula I over a measurement time window. The spectral energy is emitted by the compound of Formula I in response to electromagnetic radiation delivered to the compound of Formula I. The method also includes determining the GFR in said patient based on the measured spectral energy emitted by the compound of Formula I over the measurement time window by fitting an exponential function to the spectral energy as a function of time or a linear function to the log of the spectral energy as a function of time to calculate a rate constant associated with renal clearance over the measurement time window and directly related to the GFR normalized to a body size metric of the patient.

Transducers, kits, systems, and methods for determining a concentration of an analyte are described. In an embodiment, the transducers include a chromophore; and an enzyme physically associated with the chromophore. In an embodiment, the transducer is configured to catalyze a reaction comprising a plurality of reaction elements. In an embodiment, the plurality of reaction elements comprises one or more reactants including the analyte and one or more products. In an embodiment, an amount of fluorescence emitted from the chromophore is determined by a concentration of a reaction element of the plurality of reaction elements.

A near-infrared fluorescent small-molecule probe, a synthesis method thereof, and an application thereof are provided. The near-infrared fluorescent small-molecule probe is IR-780-F derived from IR-780, in which the basic structure of IR-780 is retained, an end of an alkyl carbon chain connected with a nitrogen (N) atom is changed by adding a trifluoromethyl group (—CF.sub.3) at the end. The IR-780-F shows excellent performance in photostability, targeting cancer cells and fluorescence imaging, and has low toxicity. It can be used for targeting tumor tissues, in vivo near-infrared fluorescence imaging, and photothermal therapy, which realizes an integration of diagnosis and treatment.

Systems, apparatuses, and methods are described for 3D luminescence imaging, by identifying a preferred optical pair and optimizing a scanned image using the preferred optical pair. An optimal filter pair may be selected from a list of two or more optical filters. An acceptable threshold of information may be obtained using a subset of the list of two or more optical filters (e.g., an optimal filter pair). An imaging device may be configured with the optimal filter pair to produce a pair of luminescence images of a target sample. In addition, luminescence images may be pre-processed to reduce the time-cost of conventional processing techniques of luminescence images. One or more computing devices may generate initial prior data based on a pair of luminescence images. An output may include one or more output luminescent sources that have been refined and/or optimized from the initial prior data.

One aspect of the invention relates to a method for intraoperative detection of parathyroid gland viability in a surgery, comprising obtaining speckle contrast images of a parathyroid gland of a patent; and displaying the speckle contrast images of the parathyroid gland in real-time.

Optical imaging or spectroscopy described can use laminar optical tomography (LOT), diffuse correlation spectroscopy (DCS), or the like. An incident beam is scanned across a target. An orthogonal or oblique optical response can be obtained, such as concurrently at different distances from the incident beam. The optical response from multiple incident wavelengths can be concurrently obtained by dispersing the response wavelengths in a direction orthogonal to the response distances from the incident beam. Temporal correlation can be measured, from which flow and other parameters can be computed. An optical conduit can enable endoscopic or laparoscopic imaging or spectroscopy of internal target locations. An articulating arm can communicate the light for performing the LOT, DCS, or the like. The imaging can find use for skin cancer diagnosis, such as distinguishing lentigo maligna (LM) from lentigo maligna melanoma (LMM).

A system provides combined diffuse optical microscopy (DOM) and fluorescence endomicroscopy (FE). The system may use a single light source which generates structured illumination for both modalities. FE and DOM images may be displayed for qualitative assessment. Quantitative assessments may be based on both FE and DOM images. The quantitative assessments may be applied to characterize tissues. Some embodiments provide multi-spectral DOM.

The invention relates to a method for recommending cosmetics, skin care products and/or dermatological products that are adapted to the condition of the skin.