Described herein is a yeast strain wherein activity of one or more membrane transporters of the DHA1 family is reduced relative to a wild type strain or to a parental strain from which it is derived.

Described herein is a yeast strain wherein activity of one or more membrane transporters of the DHA1 family is reduced relative to a wild type strain or to a parental strain from which it is derived.

A method of producing a modified Saccharomyces cerevisiae yeast strain with enhanced resistance (or tolerance) to pretreatment-derived microbial inhibitors such as furans, phenolics and weak acids is provided, which comprises integrating at least one copy of the TAL1 gene and at least one copy of two or more of the FDH1, AR11 and ADH6 genes into the S. cerevisiae genome. A modified yeast strain so obtained is also provided, the modified yeast strain being capable of simultaneously overexpressing these genes relative to a yeast strain which hasn't been modified in the same manner. S. cerevisiae strains which have been modified as described herein can be used to ferment lignocellulosic hydrolysates containing pretreatment inhibitors such as furans, phenolics and weak acids. Suitable lignocellulosic hydrolysates include sugarcane bagasse (SCB) and waste streams from the pulp and paper industry, such as spent sulphite liquor (SSL).

The present disclosure relates to the modulation in the RAS/cAMP/PKA signaling pathway for maintaining the propagation efficiency and increasing fermentation efficiency of yeast cells. The present disclosure provides yeast cells having or engineered to exhibit a modulation in signaling in a RAS/cAMP/PKA pathway, depending on conditions. For example the yeast cells can be selected or genetically modified to express a mutated Ras1 protein, a mutated Ras2 protein, a mutated Ira1 protein and/or a mutated Ira2 protein, optionally in combination with specific promoters. Also provided herewith are methods for propagating the yeast cells as well as using the yeast cells to generate a fermented product (such as ethanol).

The present disclosure relates to the modulation in the RAS/cAMP/PKA signaling pathway for maintaining the propagation efficiency and increasing fermentation efficiency of yeast cells. The present disclosure provides yeast cells having or engineered to exhibit a modulation in signaling in a RAS/cAMP/PKA pathway, depending on conditions. For example the yeast cells can be selected or genetically modified to express a mutated Ras1 protein, a mutated Ras2 protein, a mutated Ira1 protein and/or a mutated Ira2 protein, optionally in combination with specific promoters. Also provided herewith are methods for propagating the yeast cells as well as using the yeast cells to generate a fermented product (such as ethanol).

The present disclosure provides a composition comprising, (i) protein at a protein content of at least about 16% by dry weight, wherein at least about 50% by dry weight of the protein has an amino acid sequence encoded by one or more fungal genomes, and (ii) one or more materials selected from the group consisting of a flour, an oil, a flavoring agent, and a nutritional supplement, wherein the composition has a water content that is less than or equal to about 30% water by weight.

The present disclosure provides a composition comprising, (i) protein at a protein content of at least about 16% by dry weight, wherein at least about 50% by dry weight of the protein has an amino acid sequence encoded by one or more fungal genomes, and (ii) one or more materials selected from the group consisting of a flour, an oil, a flavoring agent, and a nutritional supplement, wherein the composition has a water content that is less than or equal to about 30% water by weight.

Processes for producing ethanol comprise saccharifying cellulosic material with a cellulolytic enzyme composition and fermenting the saccharified cellulosic material with a fermenting microorganism to produce ethanol. The fermenting organism is Saccharomyces cerevisiae CIBTS1260 (deposited under Accession No. NRRL Y-50973 at the Agricultural Research Service Culture Collection (NRRL), Illinois 61604 U.S.A.) or a fermenting organism that has properties that the same or about the same as that of Saccharomyces cerevisiae CIBTS1260).

Processes for producing ethanol comprise saccharifying cellulosic material with a cellulolytic enzyme composition and fermenting the saccharified cellulosic material with a fermenting microorganism to produce ethanol. The fermenting organism is Saccharomyces cerevisiae CIBTS1260 (deposited under Accession No. NRRL Y-50973 at the Agricultural Research Service Culture Collection (NRRL), Illinois 61604 U.S.A.) or a fermenting organism that has properties that the same or about the same as that of Saccharomyces cerevisiae CIBTS1260).

A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.