A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto.

It is disclosed a process for propagating a yeast capable to ferment glucose and xylose of a lignocellulosic feedstock hydrolyzate, said process comprising propagating the yeast over at least two propagation cycles. The first propagation cycle comprises the steps of: contacting the yeast at a starting yeast density with a first cultivation medium comprising a first portion of the lignocellulosic feedstock hydrolyzate; and allowing the yeast to propagate to create a first populated broth comprising water and a first propagated yeast, wherein at least 50% of the glucose and less than 20% of the xylose in the first cultivation medium are consumed in the first propagation cycle. The second cycle comprises the steps of: separating the first populated broth in at least a first removed portion and a first residual portion, wherein both the first residual portion and the first removed portion comprise some of the first propagated yeast; contacting the first residual portion with a second cultivation medium comprising a second portion of the lignocellulosic feedstock hydrolyzate; and allowing the yeast to propagate to create a second populated broth comprising water and a second propagated yeast, wherein at least 50% of the glucose and less than 20% of the xylose in the second cultivation medium are consumed in the second propagation cycle.

It is disclosed a process for propagating a yeast capable to ferment glucose and xylose of a lignocellulosic feedstock hydrolyzate, said process comprising propagating the yeast over at least two propagation cycles. The first propagation cycle comprises the steps of: contacting the yeast at a starting yeast density with a first cultivation medium comprising a first portion of the lignocellulosic feedstock hydrolyzate; and allowing the yeast to propagate to create a first populated broth comprising water and a first propagated yeast, wherein at least 50% of the glucose and less than 20% of the xylose in the first cultivation medium are consumed in the first propagation cycle. The second cycle comprises the steps of: separating the first populated broth in at least a first removed portion and a first residual portion, wherein both the first residual portion and the first removed portion comprise some of the first propagated yeast; contacting the first residual portion with a second cultivation medium comprising a second portion of the lignocellulosic feedstock hydrolyzate; and allowing the yeast to propagate to create a second populated broth comprising water and a second propagated yeast, wherein at least 50% of the glucose and less than 20% of the xylose in the second cultivation medium are consumed in the second propagation cycle.

The present disclosure relates generally to detection of contamination of a sample or diagnosis of subject based upon detection or quantification of amino acid sequences in a sample, specifically to the identification and use of molecular biomarkers for Candida albicans biofilm infections.

The present disclosure relates generally to detection of contamination of a sample or diagnosis of subject based upon detection or quantification of amino acid sequences in a sample, specifically to the identification and use of molecular biomarkers for Candida albicans biofilm infections.

The invention features fragments of the Candida cell surface proteins Als3 and Hyr1 and combinations thereof useful in immunizing a subject against fungal or bacterial infections or both.

The invention features fragments of the Candida cell surface proteins Als3 and Hyr1 and combinations thereof useful in immunizing a subject against fungal or bacterial infections or both.

The invention features fragments of the Candida cell surface proteins Als3 and Hyr1 and combinations thereof useful in immunizing a subject against fungal or bacterial infections or both.

The invention features fragments of the Candida cell surface proteins Als3 and Hyr1 and combinations thereof useful in immunizing a subject against fungal or bacterial infections or both.

Intergenic non-coding RNA molecules that regulate the expression of HWP1 and ALS3 of Candida are provided as are methods of using the non-coding RNA molecules and complementary molecules thereof in modulating HWP1 or ALS3 expression; adherence, yeast-to-hyphal transition, or biofilm development of Candida; and preventing or treating candidiasis.