Methods for preparing and purifying 2-monoacylglyceride compounds are disclosed. In one method, an unsaturated triglyceride is reacted with water, a C.sub.1-C.sub.8 alcohol, or a mixture thereof in the presence of a lipase to produce a mixture comprising a 1,3-dihydroxy-2-monoacylglyceride and fatty esters or acids. Reaction of the 1,3-dihydroxy-2-monoacylglyceride with an aldehyde or ketone gives a mixture comprising a 2-monoacylglyceride acetal or ketal. Fatty esters or acids are removed from the mixture as an overhead product by distillation or wiped-film evaporation to isolate a purified 2-monoacylglyceride acetal or ketal. The inventive methods provide a 2-monoacylglyceride protected at the 1- and 3-positions such that the acyl unit remains at the 2-position. The products are enriched in unsaturated fatty acid content when compared with the unsaturated fatty acid content of the original unsaturated triglyceride. Each method utilizes a practical purification scheme that avoids the scale-up or toxicity issues of commonly employed purification strategies.

The disclosure relates to flexible wax compositions and a method of making the same. Described herein is a wax composition containing about 20 wt % to about 45 wt % monoacylglycerides, about 28 wt % to about 40 wt % diacylglycerides, and about 10 wt % to about 45 wt % acylglyceride polymers, which contain one or more dimerized fatty acid residue and a plurality of glycerol moieties. The flexible wax composition described herein has an average creep stiffness of less than about 30 MPa and can be used in candles, paper coatings, box coatings, fruit coatings, broadsizing for OSB, tire and rubber, polyvinyl chloride piping, crayons, and personal care applications.

The present invention has an object of providing a modified lipase having excellent stability and reactivity at a high temperature. Provided is a modified lipase with improved reactivity and/or stability at a high temperature, the modified lipase having an amino acid sequence including one or more amino acid substitutions which are selected from the group consisting of T130C-S153C, A249P, F259Y, S282P, S283Y and S300P in the amino acid sequence represented by SEQ ID NO: 1, or an amino acid sequence having 90% or more sequence identity therewith.

Compositions and methods are provided for incorporating processed oils with high monoacylglyceride (MAG) content into products and food products. Methods are specifically provided for generating high MAG content processed oils.

The present invention generally relates to oil compositions and methods of producing such oil compositions. More particularly, the present invention relates to an oil composition recovered from a fermentation product as well as methods of recovering such oil compositions for use in various processes such as bio-diesel production as well as in various edible applications.

The present invention generally relates to oil compositions and methods of producing such oil compositions. More particularly, the present invention relates to an oil composition recovered from a fermentation product as well as methods of recovering such oil compositions for use in various processes such as bio-diesel production as well as in various edible applications.

A microorganism oil composition enriched with eicosapentaenoic acid or with docosahexaenoic acid mainly in the form of diglycerides, and method for obtaining same. A microorganism oil composition includes an eicosapentaenoic acid and/or docosahexaenoic acid content greater than or equal to 500 mg/g of composition, and, with respect to the total quantity of glycerides, a quantity of diglycerides greater than 45% and a quantity of monoglycerides and diglycerides greater than 60%.

The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.

The current invention relates to a method for isolating polyunsaturated fatty acids containing lipids from lipid-containing cells.

The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.