The present invention relates to methods of producing oil raw material for HVO production from vegetable oil which has been processed by an enzyme catalysed hydrolysis process and separation to reduce the content of phosphorous.

Disclosed herein is a method for producing a raw material having foam-forming properties and/or emulsion-forming properties by causing a lipase to act on an oil/fat in a low moisture state; and a product thereof. As disclosed, an oil/fat, or an oil/fat and a carbohydrate, is reacted with a lipase in a low moisture state, that is, in a state in which a moisture content relative to a dry weight of the oil/fat is 4 to 400 [d.b.%] (an added quantity of water is 2 to 200 L relative to 50 mg of the oil/fat), thereby producing a carbohydrate-oil/fat-lipase reaction product of a precursor material having latent foam-forming properties and emulsion-forming properties, and an alkaline component or material is added to the precursor material having latent foam-forming properties and emulsion-forming properties, and the obtained mixture is foamed, emulsified or powdered, thereby producing a product in which the entire reaction product can be used as a food material. Also disclosed is a carbohydrate-oil/fat-lipase reaction product of a precursor material having latent foam-forming properties and emulsion-forming properties can be produced; and a powdered and stabilized carbohydrate-oil/fat-lipase reaction product having foam-forming properties and/or emulsion-forming properties, and a product in which the entire reaction product can be used as a food material.

The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

Vegetable oil compositions having an ethyl ester content of from 0 to 18 weight percent of the total weight of the oil composition, and uses thereof are provided.

A method for lowering saturated fatty acid content, the method comprising concentrating polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to react with a glyceride containing a polyunsaturated fatty acid; wherein the lipase reaction is performed at a temperature of not more than 25? C.

The present disclosure provides compositions and methods for enzymatic oil degumming.

Triglyceride oils having one or more populations of asymmetric triglyceride molecules are provided. Asymmetric triglyceride molecule populations are triglyceride molecules that consist of a C8:0 fatty acid or a C10:0 fatty acid at the sn-1 position and the sn-2 position, and C16:0 or C18:0 at the sn-3 position. Another population of asymmetric triglyceride molecules are triglyceride molecules that consist of a C16:0 fatty acid or a C18:0 fatty acid at the sn-1 position and the sn-2 position, and C8:0 or C10:0 fatty acid at the sn-3 position. Methods of producing triglyceride oils and using the same are provided using sucrose invertase and hydrogenation of the triglyceride oil. Triglyceride molecules are produced by using recombinant DNA techniques to produce oleaginous recombinant cells.

Methods for preparing and purifying 2-monoacylglyceride compounds are disclosed. In one method, an unsaturated triglyceride is reacted with water, a C.sub.1-C.sub.8 alcohol, or a mixture thereof in the presence of a lipase to produce a mixture comprising a 1,3-dihydroxy-2-monoacylglyceride and fatty esters or acids. Reaction of the 1,3-dihydroxy-2-monoacylglyceride with an aldehyde or ketone gives a mixture comprising a 2-monoacylglyceride acetal or ketal. Fatty esters or acids are removed from the mixture as an overhead product by distillation or wiped-film evaporation to isolate a purified 2-monoacylglyceride acetal or ketal. The inventive methods provide a 2-monoacylglyceride protected at the 1- and 3-positions such that the acyl unit remains at the 2-position. The products are enriched in unsaturated fatty acid content when compared with the unsaturated fatty acid content of the original unsaturated triglyceride. Each method utilizes a practical purification scheme that avoids the scale-up or toxicity issues of commonly employed purification strategies.

The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.

A method for producing a composition containing a DHA-containing glyceride, including a step of obtaining a composition containing a DHA-containing glyceride, in which an esterification reaction is conducted by reacting a lipase with a raw material oil and fat containing highly unsaturated fatty acid as a constituent fatty acid in the presence of water and a lower alcohol to give a composition containing a DHA-containing glyceride, wherein a reaction liquid in the reaction has a water content of 0.4% by mass or more, and wherein the composition containing a DHA-containing glyceride has an acid value of 12 or less.