An evaluation device includes a state determination unit which is configured to determine a state of cells which are an observation target under non-standard conditions on the basis of information acquired from an image of cells under standard conditions.

To show a highly accurate cell measurement method. A cell measurement method comprises: a step of staining a cultured target cell with a dye; a step of obtaining a first image and a second image which are transmission images for a first light and a second light to which the dye has different absorbance; a step of dividing each of the first image and the second image into a plurality of divided regions and comparing the first image and the second image for each of the divided regions so as to eliminate noises; and a step of integrating an indicator of a cell amount in each of the divided regions in the images from which the noises were eliminated so as to evaluate a target cell amount.

An evaluation device includes a state determination unit which is configured to determine a state of cells which are an observation target under non-standard conditions on the basis of information acquired from an image of cells under standard conditions.

To show a highly accurate cell measurement method. A cell measurement method comprises: a step of staining a cultured target cell with a dye; a step of obtaining a first image and a second image which are transmission images for a first light and a second light to which the dye has different absorbance; a step of dividing each of the first image and the second image into a plurality of divided regions and comparing the first image and the second image for each of the divided regions so as to eliminate noises; and a step of integrating an indicator of a cell amount in each of the divided regions in the images from which the noises were eliminated so as to evaluate a target cell amount.

Provided is a bacterium number measuring system comprising a database storing known bacterial growth patterns in advance and an analyzing part. The analyzing part has first cultures containing a bacterial liquid that contains a measurement target bacteria and second cultures being different from the first cultures and containing the bacterial liquid and an antimicrobial drug. The analyzing part performs bacterium number measurement which measures the number of bacteria in the first cultures on a culturing part where culturing has started in the first and second cultures. The analyzing part compares the bacterium number measurement result with the growth curves stored in the database and thereby determines a timing to perform MIC measurement which measures the number of bacteria in the second cultures to determine a minimum inhibitory concentration of the measurement target bacterium. The analyzing part performs the MIC measurement at the thus-determined timing.