A method for improving efficiency of anaerobic fermentation in a medium temperature-high temperature transition zone includes: After being subjected to heat exchange through a front heat exchanger, hydrolyzed slurry is pumped to a bottom of an anaerobic tank. A temperature of the slurry is controlled at 45±0.2° C. A central agitator is turned on after feeding is completed, to slowly gently stir the slurry in the anaerobic tank, and the central agitator is turned off after the slurry is uniformly mixed. In an operating state, making medium-temperature anaerobic flora and high-temperature anaerobic flora in methanogenic bacteria in the anaerobic sludge overlap at the fermentation temperature and be fully mixed with and in contact with the slurry, to continuously treat and convert organic substances in the slurry when the slurry is under an anaerobic fermentation condition in a medium temperature-high temperature transition zone.

Described herein are bioreactor support structures configured to be used with containers having different designs, and methods for making and using such bioreactors. The support structures described herein may include two or more agitator motors and control systems or an adjustable-position agitator motor, a removable spacer and/or lid, multiple configurations for ports and probes, and multiple exhaust filter heating blankets. Also described herein are methods of manufacturing a multi-agitator motor or adjustable-position agitator motor bioreactor, as well as methods of modifying an existing support structure to be used with a container not originally designed to be used with the existing support structure, and methods for operating these bioreactors.

A system for receiving a disposable bag (44) has a receiving container (10) with a container interior for receiving the disposable bag (44) and a temperature-control hollow wall (20) that at least partially surrounds the container interior of the receiving container (10). A temperature-control unit controls the temperature of the container interior by will a temperature-control medium arranged in the temperature-control hollow wall (20) at a maximum pressure of about 1 bar.

A scalable biodigester plant for generating biogas from livestock liquid manure is provided having a concentration tank with a stirrer associated with a pretreatment tank and treatment tanks, a liquid manure pipe, with a heating pipe communicating the tanks with a heater/generator, a biogas pipe communicating the tanks with the heater/generator, and a discharge pipe communicating the tanks with the solid/liquid separator, the tanks being movable and consisting of 20′ or 40′ maritime transport containers open in the upper part, and an expandable rubber dome that seals the upper part allowing only the passage of the biogas pipe, the containers being covered internally with a thermally insulating layer that is, in turn, covered with a reinforcement layer.

Reactor for enzymatic hydrolysis of a raw material comprising in sequence: i)—a first heat exchanger adapted to heat the raw material supplied to the reactor to a temperature within a range that favours enzymatic hydrolysis, ii)—a reactor comprising plural in reactor chambers connected in series, separated by closable valves, iii)—a second heat exchanger adapted to heat the reaction mixture to a temperature higher than the temperature range favouring enzymatic hydrolysis, the reactor being formed with inclined tubular reactor chambers assembled to form a reactor with vertical axis, the first reactor chamber being the vertically uppermost chamber of the reactor, while at least one reactor chamber is adapted to be stirred with a through-flowing inert gas.

Described herein are biofilm bioreactors for synthesis at the interface between two liquids, and methods of using such bioreactors for the biotransformation of feedstocks into chemical products. Also contemplated is the extraction of such products.

A system for growing and reproducing microorganisms that includes a basin system that includes a number of basins, where each basin has a vertical meandering system which is formed by partitions and which can be illuminated, each basin is filled with a nutrient suspension, at least one outer wall of each basin is double-walled such that a cavity is formed, a temperature control medium for controlling the temperature of the nutrient suspension can flow through the cavity.

An apparatus is provided for bioprocessing, such as for culturing cells. The apparatus includes a bioreactor with a chamber having cells and a chamber for) temperature regulation of the cells. In some embodiments, a freezer is connected to the bioreactor for freezing the cells in the chamber, and a heater may also be provided for actively thawing the cells. Related methods are also disclosed.

Provided are a cell culture apparatus and a cell culture method which are capable of improving a temperature rising rate of a temperature rising target and enhancing a temperature uniformity of the temperature rising target. The cell culture apparatus includes: a heat transfer medium having a recess part or a plurality of hole parts in a contact surface with a culture vessel for containing a cell suspension; and an air supply port which communicates with the recess part or each of the plurality of hole parts and to which a gas is supplied.

Systems and apparatuses for producing microbe-based compositions that can be used in the oil and gas industry, environmental cleanup, as well as for other applications are provided. More specifically, apparatuses comprising two vessels for use in large-scale production of sophorolipids are provided. An apparatus can include a fermentation vessel and a collector vessel connected directly to the fermentation vessel.