The invention discloses a yeast and lactic acid bacteria combination, comprising saccharomyces and lactic acid bacteria; wherein the saccharomyces comprises Candida ethanolica B-JJ1, and the lactic acid bacteria comprise at least one of Lentilactobacillus buchneri B-JR1, Lactobacillus paracasei B-JR2, Lactobacillus zeae B-JR4, Lactobacillus plantarum B-JR5 and Lactobacillus chiayiensis B-JR6. The yeast and lactic acid bacteria combination is obtained by the processes of inoculum extraction, strain inoculation, combined bacteria optimization and combined bacteria domestication. The fermentation of the yeast and lactic acid bacteria combination provided by the invention can be adopted to prepare bio-enzyme preparation. And the prepared bio-enzyme preparation can be adopted to prepare natural, green, additive-free preservatives with strong antioxidant properties that maintain the activity of plant and animal cells.

A process for preparing phenylacetic acid is provided. The process essentially includes the steps of providing one or more yeast strains belonging to the genus Yarrowia and mutants thereof, providing a culture medium including phenylalanine, transforming phenylalanine into phenylacetic acid by fermentation of the one or more yeast strains in the culture medium, the phenylacetic acid being contained in a fermentation broth obtained by the fermentation, and isolating the phenylacetic acid from the fermentation broth.

A novel gene targeting method and a nucleotide construct for the method. The method integrates a nucleotide construct containing an interference gene in an effective gene targeting region independent of the gene by homologous recombination, thereby improving the targeting efficiency of the gene. The present invention also provides a gene targeting system for gene expression regulation and gene disruption.

The present invention relates to a method for producing a recombinant protein in yeast cells, wherein the cells are subjected to a temperature shift at a specific timepoint of the cell culture. It also relates to a method for producing a recombinant protein in yeast cells by culturing said yeast cells in a medium having a high concentration of potassium ions compared to the concentration of sulfate and/or phosphate ions.

An improved bacterial oil treatment composition or pool for handling a decommissioned oil cable, which may be laid in particular as part of a power grid in the ground. The invention further relates to a bacteria growth culture medium containing the bacterial oil treatment composition for refurbishing an oil cable and a corresponding use.

The disclosure discloses a genetically engineered strain for producing porcine myoglobin and fermentation and purification thereof, and belongs to the technical field of genetic engineering. The disclosure realizes efficient secretion and expression of porcine myoglobin by integrating the gene of porcine myoglobin in P. pastoris. On this basis, optimization of the medium and culture conditions of recombinant P. pastoris can significantly increase the titer of porcine myoglobin, so that the titer can reach 285.42 mg/L under fermenter conditions. In addition, by creatively adding different concentrations of ammonium sulfate to fermentation broth step by step, the purity of myoglobin obtained by final concentration is up to 88.0%, and the purification rate is up to 66.1%. The disclosure realizes efficient expression and high purification of porcine myoglobin from various steps such as synthesis, fermentation and purification of porcine myoglobin, and provides broad prospects for industrial production of porcine myoglobin.

A highly efficient ethanol-fermentative yeast having high efficiency in ethanol production is provided without introducing a foreign gene. The highly efficient ethanol-fermentative yeast features a fermentative yeast effectively producing ethanol from pentose and hexose and being deposited to NITE Patent Microorganisms Depositary under the accession number NITE BP-01963.

Provided are a Candida tropicalis strain Am2525, with the preservation number thereof being CCTCC NO: M 2019419, and a method for producing long-chain dicarboxylic acids by means of fermenting the strain. The method for producing the long-chain dicarboxylic acids comprises preparing a seed solution by means of the Candida tropicalis strain Am2525 and producing the long-chain dicarboxylic acids via fermentation of the seed solution. Compared with the parent, the Candida tropicalis strain Am2525 has an enhanced resistance to the toxicity of a substrate decane, improves the productivity of long-chain dicarboxylic acids, reduces the cost of production, subsequent separation and purification are simple, and the fermentation production process is easy to implement on a large scale.

The present invention relates to: a novel Pichia kudriavzevii microorganism NG7 showing heat resistance and acid resistance; a composition, for producing organic acid or alcohol, which comprises the microorganism and a culture of the same; and a method, for producing an organic acid or alcohol, which comprises culturing the microorganism.

An object of the present invention is to obtain a fermentative yeast having a highly efficient ethanol production without introducing a foreign gene. A further object is to obtain a fermentative yeast that is resistant to proliferation inhibitors such as organic acids, which prevent the growth of the fermentative yeast. Yeast having improved ethanol production ability was generated by introducing transaldolase and alcohol dehydrogenase gene by self-cloning to Meyerozyma guilliermondii that can produce ethanol effectively from pentose and hexose obtained by breeding. This fermentative yeast is deposited to NITE Patent Microorganisms Depositary under the accession number NITE ABP-01976.