Culture media comprising manganese and methods of culturing cells to improve sialylation and glycosylation of glycoproteins are provided.

The present invention relates to a method of increasing the yield of at least one of oxidosqualene, triterpenes and/or triterpenoids in a specifically engineered host cell and a respective host cell as well as to the use of said host cell for manufacturing the at least one of oxidosqualene, triterpenes and/or triterpenoids.

The present invention relates to the unexpected discovery of a novel yeast culture medium comprising a concentration of cupric sulfate sufficient to promote growth of strains of Saccharomyces cerevisiae var. diastaticus while inhibiting growth of other varieties of brewing yeast. In certain embodiments, the invention provides a yeast culture medium. In other embodiments, the invention provides methods of using the novel yeast culture medium to test yeast slurries for contamination with Saccharomyces cerevisiae var. diastaticus.

A method of creating a protein rich conditioned medium. The method includes culturing mesenchymal stem cells in a container utilizing a first growth medium, allowing a time period for proliferation of the mesenchymal stem cells until a desired level of confluence is achieved in the container, discarding a supernatant from the container, adding a second medium to the container, incubating the mesenchymal stem cells, and collecting the conditioned medium. The method produces significantly higher quantities of byproducts secreted by the mesenchymal stem cells. Byproducts are usable for wound healing, disease treatment, cosmetic, or other beneficial effects when applied or otherwise delivered to a patient.

The present invention relates to an angiogenesis-inducing method using neural stem cells. According to the present invention, neural stem cells derived from human brain tissues release MCP-1 or Gro, thus exhibiting an angiogenesis-inducing effect. When CoCl.sub.2 was treated to the neural stem cells of the present invention and when the neural stem cells were cultured in a hypoxia chamber, it was observed that a hypoxia condition increased the release amounts of MCP-1 and Gro. Therefore, the human brain tissue-derived neural stem cells of the present invention can be used to induce angiogenesis.

The present invention pertains to a cell culture medium comprising manganese as a media supplement, which was shown to control recombinant protein glycosylation and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.

Cell culture media are provided herein as are methods of using the media for cell culture and antibody production from cells. Compositions comprising antibodies and fragments thereof, produced by the methods herein are also provided.

Provided herein are, inter alia, are media compositions useful for culturing neural cells. In particular, the compositions provided herein mimic important physiological conditions in the living brain and sustain neural activity. The media compositions provided herein improve the efficiency of human neuron maturation and promote synaptic function in long-term in vitro cultures.

Growth medium are disclosed for use in fermenting a marine microorganism. The medium comprise Potassium, Calcium, Strontium, Borate and Fluoride at specific concentrations. Alternatively, the growth medium comprises cobalt at specified concentrations or comprises vitamin B.sub.12 at specified concentrations. Methods of producing certain desired compound by fermentation of a marine microorganism are also disclosed.

A medium material for removing phenol contamination from groundwater, a method of producing the same, and use of the same id disclosed. In at least some embodiments, the medium material is a granular material which has an average particle diameter of 0.5-1.5 cm and is formed from a bacteria-entrapping solution, a manganese sand filter material, modified bentonite, and biochar at a mass ratio of 1:0.2-0.4:0.2-0.4:0.1-0.2 by a series of processes including strain culturing, catalysis, mixing, solidification, and the like. The medium material can remove phenol from groundwater, is a safe and environment-friendly material, has a long service life, and/or achieves waste treatment with waste.