The present invention relates in part to methods for producing tissue-specific cells from patient samples, and to tissue-specific cells produced using these methods. Methods for reprogramming cells using RNA are disclosed. Therapeutics comprising cells produced using these methods are also disclosed.

The present invention discloses a use of mitochondria to promote wound repair and/or healing. Specifically, when a certain amount of mitochondria or a composition containing a certain amount of mitochondria is administered to a wound, the effect of promoting wound repair or accelerating wound healing can be effectively achieved.

3D native tissue-derived scaffolding materials are made in various formats, including but not limited to hydrogel, sponge, fibers, microspheres, and films, all of which function to better preserve natural extracellular matrix molecules and to recapitulate the natural tissue environment, thereby effectively guiding tissue regeneration. Tissue-derived scaffolds are prepared by incorporating a homogenized tissue-derived suspension into a polymeric solution of synthetic, natural, or hybrid polymers. Such tissue-derived scaffolds and scaffolding materials have a variety of utilities, including: the creation of 3D tissue models such as skin, bone, liver, pancreas, lung, and so on; facilitation of studies on cell-matrix interactions; and the fabrication of implantable scaffolding materials for guided tissue formation in vivo. The tissue-derived scaffolds and scaffolding materials also provide the opportunity to correlate the functions of extracellular matrix with tissue regeneration and cancer metastasis, for example.

Provided are a culture medium and cultivation method of rapidly expanding primary cells of esophageal squamous carcinoma in vitro, and the use thereof in screening drugs. The culture medium comprises an initial culture medium selected from DMEM/F12, DMEM, F12, or RPMI-1640, a Rho protease inhibitor, an antibiotic, insulin, an N2 additive, insulin-like growth factor 1, a non-essential amino acid, and optionally, hydrocortisone, optionally, glutamine, and optionally, bovine pituitary extract.

The subject matter of the present invention is a method for differentiating epithelial stem cells, comprising culturing one or more epithelial stem cells in contact with an extracellular matrix in the presence of an expansion medium, a bovine pituitary extract, a receptor tyrosine kinase ligand, a supernatant of primary fibroblasts and optionally, a Rho kinase inhibitor.

The present invention relates to a method for fabrication of an extracellular matrix-induced self-assembly and to fabrication of an artificial tissue using same. The method for fabrication of an extracellular matrix-induced self-assembly comprise the steps of: (a) decellularizing and powdering a tissue-derived extracellular matrix (ECM); and (b) adding the decellularized extracellular matrix powder to cells and culturing the cells to form a cell-extracellular matrix powder self-assembly. Accordingly, the self-assembly has characteristics similar to those of extracellular matrix tissues and can be fabricated into three-dimensional artificial tissues 1 cm or greater in size, thus finding advantageous applications as a cell therapy product and an artificial tissue implant.

The present disclosure provides a method for promoting the growth of parietal decidual basalis-mesenchymal stem cells (PDB-MSCs), which comprises promoting the growth of parietal decidual basalis-mesenchymal stem cells (PDB-MSCs) via a human umbilical cord-mesenchymal stem cell (UC-MSC)-derived exosome. In the present disclosure, after the PDB-MSCs are co-cultivated with the human UC-MSC-derived exosome, the PDB-MSCs show strong cell proliferation ability, prominent cell shape, and desirable cell viability. That is, the human UC-MSC-derived exosome of the present disclosure can improve a quality of PDB-MSCs and effectively improve the ability of PDB-MSCs to secrete vascular endothelial growth factor (VEGF) and stem cell factor (SCF), so as to solve the problem that PDB-MSCs show decreased proliferation ability and poor cell viability after multiple passages, which effectively facilitates the large-scale cultivation and clinical practice of PDB-MSCs.

The present invention relates to a composition for inducing browning, a pharmaceutical composition for preventing or treating metabolic diseases, and a food composition for alleviating metabolic diseases, all of the compositions containing milk exosomes. In addition, the present invention relates to a method for inducing the differentiation of white adipocytes into beige adipocytes or brown adipocytes by treatment with the milk exosomes, and a method for treating obesity or metabolic diseases by administering the milk exosomes.

The application provides new compositions and methods for stimulating the production of natural killer (NK) cells in a subject. NK cells can be selectively expanded with a combination of stimulating ligands. Methods and compositions for the administration of stimulatory ligands modified to self-insert into tumor cells, thereby stimulating an increase in the number of NK cells in proximity to a tumor, are also described.

The present application relates to the field of cell biology. The present application provides a cryopreservation medium for cryogenic storage of a biological sample, particularly a cryopreservation medium for cryogenic storage of cells in an apheresis sample.