This disclosure describes devices and methods used to inject or aspirate fluid into or from various regions of the human body, such as the anterior chamber of the eye. The disclosed devices and methods provide improvements over conventional devices and methods in that an operator can perform a procedure using the disclosed device without an assistant. The disclosed devices and methods allow one-handed injection or aspiration of fluid into or from body tissue, and provide means for controlling the volume of injected or aspirated fluid.

A system can facilitate pre-treatment a biofluid sample for various applications for monitoring and/or tracking a subject's health. The system includes a sample collection component that can collect the biofluid sample. The system also includes a mixing component that includes a media with a material. Upon adding the biofluid sample to the mixing component, the biofluid sample mixes with the media. The system also includes a filter component that can filter the media from the biofluid sample. After the media is filtered from the biofluid sample, the biofluid sample can be provided to an analyte analysis application.

An ocular device for insertion into the eye (for example, into a lacrimal punctum or a conjunctival sac) are described herein. The ocular device may continuously capture specific analytes from tear fluid. Several embodiments of the ocular device may have one or more open channels and pores that enable tears to drain naturally through while capturing specific analytes. A mechanism of detecting analytes in bodily fluids using materials comprised of DNAzymes and/or aptamers are described herein. Aptamers are oligonucleotide or peptide molecules that bind to a specific target molecule. DNAzymes are designed to catalyze a number of biological reactions (i.e., RNA cleavage, DNA cleavage, ligation, or phosphorylation reactions). A number of ways an ocular insert may capture analytes in tear fluid in vivo for subsequent in vitro analysis are described herein. Additionally, a specific mechanism for colorimetric detection of analytes using aptamer- and/or DNAzyme-crosslinked hydrogels are described herein.

An embodiment of the present disclosure provides a device for stimulating tear secretion including a container body including a rim capable of coming into close contact with a face in a region around an eye, the container body defining therein a gas-receiving space capable of being filled with gas generated by an eye irritant.

A lacrimal tear flow measurement device, and methods of manufacture and use, are described that includes a polymer microcapillary tube or similar structure having at least one end coated on the outside with soft silicone rubber and one end treated on the inside to be hydrophobic. The hydrophobic end keeps liquid from escaping or entering that end while allowing air to pass. The rest of the tube's insides may be hydrophilic or a neutral hydrophobe. As a Schirmer's test strip replacement, the entrance end of the device can be touched to the lacrimal lake of a patient's eye to collect suck up, or merely collect, tear fluid within the collection tube for weighing, volume measurement, or other analysis. Long-term collection devices for wear between doctors' visits can have a bypass channel allowing liquid to flow back onto the eye.

Provided herein are devices, systems, and methods for biological sample collection. In particular, provided herein are devices, systems, and methods that employ small, hand-held devices that collect small volumes of biological samples, such as vitreous from the eye, for diagnostic and other purposes.

An article of manufacture comprises an optical shade configured for removable attachment to eyewear, e.g., eyeglasses that are retrofitted to include a micro-fluidic tear collector in a nose pad. The optical shade comprises a lens having a lateral flow assay comprising an antibody having an associated fluorescent tag. The fluorescent tag is configured to fluoresce and change a color of the lens upon detection by the flow assay of an antigen that matches the antibody. The antigen comprises viral constituents, such as SARS-CoV-2. The color change of the lens is visible and indicates presence of Covid-19 infection.

An auto-powered biosensor capable detecting a target molecule, and a method of powering the same, wherein the biosensor is fabricated with a microfluidics layer, a multimodal sensing layer comprising a biofuel cell and an electrode, and a logic circuit that may include a processor and non-transitory memory with computer executable instructions embedded thereon.

An article of manufacture comprises an optical shade configured for removable attachment to eyewear, e.g., eyeglasses that are retrofitted to include a micro-fluidic tear collector in a nose pad. The optical shade comprises a lens having a lateral flow assay comprising an antibody having an associated fluorescent tag. The fluorescent tag is configured to fluoresce and change a color of the lens upon detection by the flow assay of an antigen that matches the antibody. The antigen comprises viral constituents, such as SARS-CoV-2. The color change of the lens is visible and indicates presence of Covid-19 infection.

A system can facilitate pre-treatment a biofluid sample for various applications for monitoring and/or tracking a subject's health. The system includes a sample collection component that can collect the biofluid sample. The system also includes a mixing component that includes a media with a material. Upon adding the biofluid sample to the mixing component, the biofluid sample mixes with the media. The system also includes a filter component that can filter the media from the biofluid sample. After the media is filtered from the biofluid sample, the biofluid sample can be provided to an analyte analysis application.