The present invention provides mRNAs usable as vaccines against lassa virus (LASV) infections. Further, the Invention relates to (pharmaceutical) compositions and vaccines comprising said mRNAs and their use for treatment or prophylaxis of a lassa virus infection. The present invention further features a kit comprising the mRNAs, (pharmaceutical) compositions or vaccines and a method for treatment or prophylaxis of lassa virus infections using said mRNAs, (pharmaceutical) compositions or vaccines.

Provided herein are, inter alia, methods and compositions for treating and preventing arenavirus infection. Compositions include engineered arenavirus glycoproteins that are able to form glycoprotein trimers. The glycoprotein timers are contemplated to be effective for preventing and/or treating arenavirus infections.

The invention relates to recombinant VSV viruses and viral vectors which produce a glycoprotein GP of the lymphocyte choriomeningitis virus (LCMV) instead of the G protein of the VSV, to virus producing cells which produce LCMV-GP-pseudotyped VSV virions, and to the use of said vectors and cells in the therapy of solid tumors, especially brain tumors.

The present invention relates to the field of oncolytic viruses and in particular to a recombinant rhabdovirus, such as vesicular stomatitis virus encoding in its genome for a CD80 extracellular domain Fc-fusion protein. The invention is further directed to the use of the recombinant virus in the treatment of cancer, and also to methods for producing such viruses.

The invention relates to recombinant VSV viruses and viral vectors which produce a glycoprotein GP of the lymphocyte choriomeningitis virus (LCMV) instead of the G protein of the VSV, to virus producing cells which produce LCMV-GP-pseudotyped VSV virions, and to the use of said vectors and cells in the therapy of solid tumors, especially brain tumors.

The invention relates to genetically modified arenaviruses suitable for the treatment of neoplastic diseases, such as cancer. The arenaviruses described herein may be suitable for treatment of neoplastic diseases and/or for the use in immunotherapies. In particular, provided herein are methods and compositions for treating a neoplastic disease by administering a genetically modified arenavirus, wherein the arenavirus has been engineered to include a nucleotide sequence encoding one or more antigenic fragment(s) of mutant KRAS alone or to further include a nucleotide sequence encoding one or more antigenic fragment(s) of a mutated cancer driver gene (e.g., a mutant TP53) or a tumor-associated antigen.

The invention relates to methods of treating or preventing a tumor.

The present disclosure relates to a novel live-attenuated Junn virus (JUNV), which exhibits reduced likelihood for virulence reversion compared to the Candid#1 vaccine strain. The live-attenuated JUNV comprises at least three mutations in its GPC protein compared to a pathogenic strain of JUNV. One mutation is a substitution mutation at residue 427 like the Candid#1 vaccine strain. The live-attenuated JUNV comprises at least one mutation altering a NxT/S glycosylation motif in a region spanning residues 150-175 of the Junn virus GPC protein to inactivate the glycosylation site. In some embodiments, the live-attenuated JUNV further comprises a substitution mutation at residue 33. Compositions comprising the variant JUNV and methods of using the JUNV and compositions are also described herein.

The invention concerns a multivalent immunogenic composition comprising recombinant live attenuated Mopeia viruses (MOPV), wherein each valence is constituted by a recombinant live attenuated Mopeia virus in which the MOPV nucleoprotein (NP) has attenuated exonuclease activity and the encoded glycoprotein precursor (GPC) is from a New World arenavirus selected from one of the following arenaviruses: Machupo virus (MACV), Sabia virus (SABV), Chapare virus (CHAPV), Junin virus (JUNV) and Guanarito virus (GTOV). The invention also concerns a combination of active ingredients, a composition or vaccine, or a therapeutically effective composition, comprising such recombinant live attenuated Mopeia viruses (MOPV) for use in eliciting a protective immune response in a mammalian host against a New World arenavirus infection. The invention also concerns a method of preparing such recombinant live attenuated Mopeia viruses (MOPV) in a eukaryotic host cell and a method of preparing a multivalent, in particular a pentavalent, immunogenic composition comprising recombinant live attenuated Mopeia viruses (MOPV) expressing a GPC protein of a New World arenavirus selected among: Machupo virus (MACV), Sabia virus (SABV), Chapare virus (CHAPV), Junin virus (JUNV) and Guanarito virus (GTOV).

Provided herein are polynucleotides encoding Lassa virus glycoprotein or Lassa virus nucleoprotein, or a chimeric protein comprising the ectodomain of Lassa virus glycoprotein and the transmembrane and cytoplasmic domains of NDV F protein. Also, provided herein are recombinant Newcastle disease virus (NDV) comprising such a polynucleotide, and immunogenic compositions comprising such recombinant NDV. Further, provided herein are methods for immunizing against Lassa virus comprising administering the recombinant NDV or an immunogenic composition thereof.