The present disclosure provides materials and methods relating to recombinant viral expression vectors. In particular, the present disclosure provides novel recombinant viral expression vectors comprising an RNA virus backbone that encodes a virus capable of infecting a target organism and expressing a polynucleotide-of-interest in that target organism. The novel viral vector constructs provided herein are a versatile expression tool for interrogating gene function and an efficient delivery platform for gene editing technology.

The present invention relates to exosomes, loaded with genetic material and methods of producing them and to the use of such exosomes for delivering genetic material in vivo, in particular the use of such exosomes in methods of gene therapy or gene silencing.

The present invention belongs to the field of compliance markers and marker vaccines which allow for the differentiation between infected and vaccinated individuals. In particular, it relates to a method of determining whether an individual has received an immunogenic composition comprising a recombinant protein produced by a baculovirus expression system in cultured insect cells.

The disclosure provides artificial viral compositions for use in treating cancer or a hyperproliferative disorder in a subject to whom the compositions are administered, as well as to methods of making and using the compositions.

The present invention belongs to the field of compliance markers and marker vaccines which allow for the differentiation between infected and vaccinated individuals. In particular, it relates to a method of determining whether an individual has received an immunogenic composition comprising a recombinant protein produced by a baculovirus expression system in cultured insect cells.

Provided herein are chimeric pseudotyped recombinant RABV particles, wherein the chimeric envelope protein comprises, from N-terminus to C-terminus, a non-RABV envelope protein ectodomain, a non-RABV envelope protein transmembrane domain, and a RABV C-terminal domain. Also provided are methods for their use in delivering a transgene into a target cell.

Herein, is describe a new technology that exploits modified-virus-like particles (VLP) to improve deliverability and increase effectiveness of dsRNAs/chemicals/peptides to difficult-to-control phytophagous insect pests, including hemipteran and lepidopteran insects. This technology has also the potential to control plant pathogens including fungi, like Asian Soybean Rust (ASR).

The present invention relates to exosomes, loaded with genetic material and methods of producing them and to the use of such exosomes for delivering genetic material in vivo, in particular the use of such exosomes in methods of gene therapy or gene silencing.

Disclosed is a system for targeting expression of antigens delivered via DNA vaccines to cell surface. Antigens, vaccines, and methods of using of these vaccines in prevention of fish from infections are also disclosed.

Disclosed are salmon interferon sequences useful as adjuvants in fish vaccines. Also disclosed are fish vaccines utilizing said interferon sequences and methods for prevention of fish infections utilizing these vaccines.