A device for sampling microorganisms comprising a hollow probe that can be inserted into a growth substrate or water system, the probe containing a growth medium for microorganisms wherein the growth medium contains a chemoattractant particularly a plant pathogen chemoattractant wherein the growth medium is specific for the microorganism/microorganisms which a grower wishes to know if they are present in the growth substrate or water system.

A method, a medium and a kit for the enrichment and detection of Listeria species, especially Listeria monocytogenes. The medium is an enrichment medium of C12 to C16 fatty acids and/or derivatives thereof.

The present invention provides a novel method for more simply and rapidly detecting target bacterial cells by a device using a binding molecule capable of binding to the target bacterial cells. In the method, a sample is incubated in a reagent for concentration of bacteria to cause the sample to react with a fluorescently-labeled binding molecule, and a fluorescence polarization degree is then detect to detect the target, and this is performed using a bacterial detection tool. The bacterial detection tool is obtained by attaching a sample collection tool to a main body. The sample collection tool includes a collection section and a connection section that is connected to the main body. The main body includes a reagent storage chamber, a collection section storage chamber, and a connection section that is connected to the sample collection tool. The reagent storage chamber and the collection section storage chamber are separated from each other. The sample collection tool and the main body are connected to each other at the connection section of the sample collection tool and the connection section of the main body after the preparation step and before the incubation step with the collection section being placed inside the collection section storage chamber. The reagent storage chamber and the collection section storage chamber internally communicate with each other after the preparation step to perform the incubation step and the reaction step. h a sample is collected; and a connection section that is connected to the main body, the main body comprises: a reagent storage chamber that contains the reagent and the fluorescently-labeled binding molecule; a collection section storage chamber that contains the collection section of the sample collection tool; and a connection section that is connected to the sample collection tool, the reagent storage chamber and the collection section storage chamber are separated from each other, and the sample collection tool and the main body are connected to each other at the connection section of the sample collection tool and the connection section of the main body with the collection section of the sample collection tool being placed inside the collection section storage chamber of the main body.

Culture medium devices and systems are shown and described. In one embodiment, the device comprises a culture medium adapted for test fluid inoculation without the concerns associated with a spreading step. In particular examples, a printed grid on the outer surface of a culture device is visible on the inner surface for colony counting after a test has been developed. The result is a device that allows for detection, identification, and transportation of various microorganisms without preparation or spreading steps, and more particularly to a culture medium in which a test fluid inoculated thereto diffuses rapidly.

Methods and systems are for identifying the cell type of an unknown microorganism. The device includes: an apparatus for culturing unknown organism(s), a diagnostic data acquisition tool and a computer program. The method includes: incubation of the sample with a growth medium (with or without toxins), and an analysis of the metabolites detected in the sample. The computer system compares the results collected from the device to reference metabolite profiles.

Methods, compositions, and kits are provided for rapidly analyzing microbial growth and/or number in a plurality of water-in-oil emulsion droplets.

The present disclosure discloses a medium for primary isolation of Porphyromonas gingivalis, and a method for preparing the medium and use thereof. The medium for primary isolation of Porphyromonas gingivalis includes the following components in parts by weight: 10-20 parts of a mixed peptone, 5-10 parts of a yeast extract powder, 1-5 parts of sodium chloride, 10-15 parts of agar, 0.5-1.0 parts of glucose, 0.2-0.8 parts of sodium bicarbonate, 0.1-0.5 parts of an L-cysteine salt, 0.1-0.5 parts of soluble sodium pyrophosphate, 0.0001-0.0005 parts of hemin, 0.00001-0.00005 parts of vitamin K, 500-1000 parts of water and 5-10 parts of a sterile defiberized sheep blood. By adopting the medium for primary isolation of Porphyromonas gingivalis provided by examples of the present disclosure, a culture period of primary isolation of Porphyromonas gingivalis can be greatly shortened, and the primary isolation of Porphyromonas gingivalis can be quickly conducted.

This disclosure describes bacteriophages that may be used to improve the selective isolation of Campylobacter from foods or to control antibiotic-resistant bacteria, or both. This disclosure further describes methods of using those bacteriophages.

The present document is directed to a method for the detection and/or enumeration of Listeria bacteria, such as Listeria monocytogenes in a sample, said method comprising culturing a sample possibly containing Listeria bacteria in a culture medium comprising rhamnose, one or more of an antibiotic, a pH colour indicator and LiCl.

This invention relates to a method for the direct detection of tick-borne infections, wherein the human/animal body fluid sample, ideally blood sample to be examined is obtained directly on a specified amount of cell technology medium establishing a partially hostile (semi-hostile) environment, the sample and the cell technology medium are mixed, incubated and stored, if required, meanwhile, the cells and pathogens of the subject are in the semi-hostile environment, then corpuscles and pathogens are separated; a small amount of blood cells from the “buffy coat” layer and blood cells located a few cell layers beneath it are supplemented to and mixed with the gained plasma, the sample is concentrated and the test is finally performed to detect pathogens in the plasma and in blood cells.

The claimed matter covers cell technology medium applied and its application interesting methods.