Provided is a method for detecting and counting the relative content of a microorganism, comprising: adding a redox indicator to a growth medium to produce an indicating growth medium; the range of color variation of the redox indicator comprising three or more colors that can be easily recognized by the naked eye; diluting a sample to be tested, configuring multiple degrees of dilution, configuring multiple parallels for each degree of dilution, and growing the diluted test sample using the indicating growing medium; reading the color or absorbance of the indicating growth medium while growing and/or when growing is completed; and producing the relative content of a microorganism in the test sample on the basis of the level of color variation or the value of absorbance variation of the indicating growth medium.

The present disclosure relates to a consumable sample partition device and it assembly and use. The sample partition device can be used to test a sample for absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). The sample partition device partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.

The present disclosure relates to a consumable sample partition device and it assembly and use. The sample partition device can be used to test a sample for absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). The sample partition device partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.

Methods for processing and analyzing samples are presented, said methods useful for, among other uses, analysis of lipids and other analytes of a sample, said methods further useful for identification of microorganisms at the level of species, identification of microorganisms at a level other than species, detecting infections and other diseases, detecting and measuring growth of an organism, detecting and measuring an environmental response of an organism, determining and/or measuring antimicrobial resistance of a microorganism, and for other purposes.

Methods for processing and analyzing samples are presented, said methods useful for, among other uses, analysis of lipids and other analytes of a sample, said methods further useful for identification of microorganisms at the level of species, identification of microorganisms at a level other than species, detecting infections and other diseases, detecting and measuring growth of an organism, detecting and measuring an environmental response of an organism, determining and/or measuring antimicrobial resistance of a microorganism, and for other purposes.

Cell counting device A cell counting device and a method of using a cell counting device are disclosed. The cell counting device comprises a chamber for receiving a sample, at least one light source to emit light towards a section of the chamber. The section of the chamber comprises a sub-sample of the sample. The cell counting device also comprises a light detector to receive a light emitted from the section of the chamber and to generate an electronic signal associated with the received light, and a controller. The controller is configured to estimate the number of photoactive cells in the sample by calculating the distribution of variable fluorescence [F.sub.v] values of a predetermined number of sub-samples about the mean F.sub.v value.

The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.

The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.

According to the present invention, a somatic cell meter includes a somatic cell concentration measuring section, a time constant recording section, and a somatic cell concentration deriving section. The somatic cell concentration measuring section measures the somatic cell concentration of centrifuged raw milk in association with the duration of the centrifugation. The time constant recording section records a time constant in the association relationship between the somatic cell concentration and the duration. The somatic cell concentration deriving section derives the somatic cell concentration based on a measurement result from the somatic cell concentration measuring section and a recorded content from the time constant recording section.

Methods of culturing and detecting a biomarker which may be associated with autoimmune diseases, in particular inflammatory bowel disease and Crohn's disease; also a screening method for substances suitable for the treatment of inflammatory bowel disease including Crohn's disease; the method including culturing a biomarker in a culture media which includes a culture broth, OADC, PANTA and Mycobactin J.