A platform and method for conducting multi-variable combinational interactions are provided. An array of multiplexing chambers in formed in a body. The body also includes a common well communicating with each multiplexing chamber of the array of multiplexing chambers and a plurality of variable wells. Each of variable wells communicates with at least one multiplexing chamber of the array of multiplexing chambers. The common well is loaded with a first variable and different variables are loaded in each of the plurality of variable wells. The interaction of the first variable with at least one of the different variables in each multiplexing chamber of the array of multiplexing chambers is observed.

The subject invention provides methods of producing advantageous microbes and/or microbial growth by-products using a naturally-derived biostimulant composition. Specifically, the subject invention provides methods for producing bacteria, such as Bacillus spp. bacteria, wherein the rate of cell growth is increased through the application of a biostimulant composition comprising peanut hearts to the cultivation medium.

The subject invention provides methods of producing advantageous microbes and/or microbial growth by-products using a naturally-derived biostimulant composition. Specifically, the subject invention provides methods for producing bacteria, such as Bacillus spp. bacteria, wherein the rate of cell growth is increased through the application of a biostimulant composition comprising peanut hearts to the cultivation medium.

Disclosed are methods and systems for rapidly testing antimicrobial susceptibility, wherein the qualitative and quantitative susceptibility of an inoculated microorganism against an antimicrobial agent or a combination of antimicrobial agents is determined as a function of one or more slopes of linear trends α of data of readouts.

Disclosed are methods and systems for rapidly testing antimicrobial susceptibility, wherein the qualitative and quantitative susceptibility of an inoculated microorganism against an antimicrobial agent or a combination of antimicrobial agents is determined as a function of one or more slopes of linear trends α of data of readouts.

A method for detecting a presence or an absence of at least one zone of inhibition, the method including a step consisting in depositing a volume of the sample in liquid form along a deposition zone extending along an axis at the surface of the agar culture medium and a step consisting in depositing a determined amount of a chemical agent at the surface of the agar culture medium, the deposit defining a potential zone of inhibition, the axis of the zone of deposition of the sample intersecting the potential zone of inhibition.

A method for detecting a presence or an absence of at least one zone of inhibition, the method including a step consisting in depositing a volume of the sample in liquid form along a deposition zone extending along an axis at the surface of the agar culture medium and a step consisting in depositing a determined amount of a chemical agent at the surface of the agar culture medium, the deposit defining a potential zone of inhibition, the axis of the zone of deposition of the sample intersecting the potential zone of inhibition.

The present invention relates to a method of interpreting different antibiogram images in which it is possible to recognize a phenotype of bacterial resistance relative to antibiotics by comparing photographs using a photographic image bank of the reference antibiogram without any need to interpret them using the EUCAST or CA-SFM interpretation data; providing that for a given phenotype, there is available a collection of photographs of a plurality of bacteria of the same species and of the same phenotype.

The present invention relates to a method of interpreting different antibiogram images in which it is possible to recognize a phenotype of bacterial resistance relative to antibiotics by comparing photographs using a photographic image bank of the reference antibiogram without any need to interpret them using the EUCAST or CA-SFM interpretation data; providing that for a given phenotype, there is available a collection of photographs of a plurality of bacteria of the same species and of the same phenotype.

A device for acquisition of particles present in a sample includes a spatially coherent light source, an optical system, and an image sensor placed in the focal plane of the optical system. The image sensor is configured to capture an intensity image. A computational unit of the device is configured to construct a series of electromagnetic propagation matrices obtained for a plurality of defocusing offsets relative to a plane of focus of the optics. The computational unit is also configured to determine a first average focused electromagnetic matrix for the particles from the series of electromagnetic matrices, identifying at least one of the particles in the first electromagnetic matrix and storing the coordinates of said particle, and determining a second electromagnetic matrix at a distance of focus on a particle identified from the components of the series of electromagnetic matrices having the stored coordinates.