An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.

A double-stranded DNA to be analyzed is analyzed without subjecting it to a modification treatment. An adapter molecule to be bound to the double-stranded DNA to be analyzed has a double-stranded nucleic acid region having base sequences complementary to each other, a pair of single-stranded nucleic acid regions having base sequences non-complementary to each other, and a block molecule placed in one of the single-stranded nucleic acid regions.

A double-stranded DNA to be analyzed is analyzed without subjecting it to a modification treatment. An adapter molecule to be bound to the double-stranded DNA to be analyzed has a double-stranded nucleic acid region having base sequences complementary to each other, a pair of single-stranded nucleic acid regions having base sequences non-complementary to each other, and a block molecule placed in one of the single-stranded nucleic acid regions.

A nucleic acid or peptide detection system that includes a carrier with at least two areas is provided. A method and kit that use and/or include the nucleic acid peptide detection system are also provided.

A nucleic acid or peptide detection system that includes a carrier with at least two areas is provided. A method and kit that use and/or include the nucleic acid peptide detection system are also provided.

The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures, such as RCPs, in the biological sample without the use of exogenously added oligonucleotide compaction probes. In some embodiments, provided herein are methods involving the use of self-hybridizing hybridizing regions for compacting and/or stabilizing nucleic acid concatemers (e.g., RCPs). In some embodiments, dynamic inter-strand annealing between tandem units of an RCP is used for compaction and/or stabilization. In some embodiments, short palindromic regions in an RCP are used for compaction and/or stabilization.

The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures, such as RCPs, in the biological sample without the use of exogenously added oligonucleotide compaction probes. In some embodiments, provided herein are methods involving the use of self-hybridizing hybridizing regions for compacting and/or stabilizing nucleic acid concatemers (e.g., RCPs). In some embodiments, dynamic inter-strand annealing between tandem units of an RCP is used for compaction and/or stabilization. In some embodiments, short palindromic regions in an RCP are used for compaction and/or stabilization.

The present invention is directed to methods for improving assay specificity and performance in binding assays.

The present invention is directed to methods for improving assay specificity and performance in binding assays.

Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one organic solvent having a dielectric constant in the range of no greater than 115; and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.