The present invention relates to methods for detecting and quantifying intact protein-polynucleotide conjugate molecules in various sample matrices. In particular, the methods utilize triplex forming oligonucleotides in combination with protein-specific binding partners to respectively detect the polynucleotide and protein components of the conjugate molecules.

The present disclosure relates to chemical compositions, kits, and apparatuses and methods for using these compositions, kits and apparatuses in various assays.

A composition includes a nanopore including first and second sides and an aperture, nucleotides each including an elongated tag, and a first polynucleotide that is complementary to a second polynucleotide. A polymerase can be disposed adjacent to the first side of the nanopore and configured to add nucleotides to the first polynucleotide based on a sequence of the second polynucleotide. A permanent tether can include a head region anchored to the polymerase, a tail region, and an elongated body disposed therebetween that occurs in the aperture of the nanopore. A first moiety can be disposed on the elongated body that binds to the elongated tag of a first nucleotide upon which the polymerase is acting. A reporter region can be disposed on the elongated body that indicates when the first nucleotide is complementary or is not complementary to a next nucleotide in the sequence of the second polynucleotide.

A composition includes a nanopore including first and second sides and an aperture, nucleotides each including an elongated tag, and a first polynucleotide that is complementary to a second polynucleotide. A polymerase can be disposed adjacent to the first side of the nanopore and configured to add nucleotides to the first polynucleotide based on a sequence of the second polynucleotide. A permanent tether can include a head region anchored to the polymerase, a tail region, and an elongated body disposed therebetween that occurs in the aperture of the nanopore. A first moiety can be disposed on the elongated body that binds to the elongated tag of a first nucleotide upon which the polymerase is acting. A reporter region can be disposed on the elongated body that indicates when the first nucleotide is complementary or is not complementary to a next nucleotide in the sequence of the second polynucleotide.

Systems and method for providing chaperone activity to a protein-containing compound is disclosed. The method includes selecting a nucleic acid based on one or more of the nucleic acid's particular properties and a specific sequence of the nucleic acid and applying the nucleic acid to a compound comprising one or more proteins to provide chaperone activity to the compound.

Peptide nucleic acids containing thymidine and 2-aminopyridine (M) nucleobases formed stable and sequence selective triple helices with double stranded RNA at physiologically relevant conditions. The M-modified PNA displayed unique RNA selectivity by having two orders of magnitude higher affinity for the double stranded RNAs than for the same DNA sequences. Preliminary results suggested that nucleobase-modified PNA could bind and recognize double helical precursors of microRNAs.

Peptide nucleic acids containing thymidine and 2-aminopyridine (M) nucleobases formed stable and sequence selective triple helices with double stranded RNA at physiologically relevant conditions. The M-modified PNA displayed unique RNA selectivity by having two orders of magnitude higher affinity for the double stranded RNAs than for the same DNA sequences. Preliminary results suggested that nucleobase-modified PNA could bind and recognize double helical precursors of microRNAs.

Peptide nucleic acids containing thymidine and 2-aminopyridine (M) nucleobases formed stable and sequence selective triple helices with double stranded RNA at physiologically relevant conditions. The M-modified PNA displayed unique RNA selectivity by having two orders of magnitude higher affinity for the double stranded RNAs than for the same DNA sequences. Preliminary results suggested that nucleobase-modified PNA could bind and recognize double helical precursors of microRNAs.

The present invention relates to a nucleic acid template for detection of a target nucleic acid based on a G-quadruplex sequence and a use thereof. A nucleic acid template for detection of a target nucleic acid or a method for detection or diagnosis of a target nucleic acid, using same according to the present invention, allows for simple and rapid detection of a target nucleic acid at room temperature with high specificity through addition and reaction of a reaction enzyme even without a separate PCR machine or a complicated temperature control procedure. In addition, the template and the method exhibit high detection sensitivity because of taking advantage of rolling circle amplification or rolling circle transcription based on circular ring formation to amplify signals, and can instantly detect a target nucleic acid even without expensive signaling substances such as fluorescent molecules, or a separate signal detection procedure such as electrophoresis because of visibly forming a gel in the presence of the target nucleic acid. Therefore, the template and the method are useful in various fields such as infectious diseases, cancer diagnosis, hereditary disease, and customized diagnosis.

The present invention relates to a nucleic acid template for detection of a target nucleic acid based on a G-quadruplex sequence and a use thereof. A nucleic acid template for detection of a target nucleic acid or a method for detection or diagnosis of a target nucleic acid, using same according to the present invention, allows for simple and rapid detection of a target nucleic acid at room temperature with high specificity through addition and reaction of a reaction enzyme even without a separate PCR machine or a complicated temperature control procedure. In addition, the template and the method exhibit high detection sensitivity because of taking advantage of rolling circle amplification or rolling circle transcription based on circular ring formation to amplify signals, and can instantly detect a target nucleic acid even without expensive signaling substances such as fluorescent molecules, or a separate signal detection procedure such as electrophoresis because of visibly forming a gel in the presence of the target nucleic acid. Therefore, the template and the method are useful in various fields such as infectious diseases, cancer diagnosis, hereditary disease, and customized diagnosis.