Improved compositions and methods for using modified non-retroviral reverse transcriptase to perform 3′ extension of a nucleic acid, employ non-templated deoxynucleotide addition to a single-stranded nucleic acid and/or synthesis of complementary DNA using non-complementary nucleic acids as primer and template (RNA- or DNA-templated DNA polymerase activity.

Improved compositions and methods for using modified non-retroviral reverse transcriptase to perform 3′ extension of a nucleic acid, employ non-templated deoxynucleotide addition to a single-stranded nucleic acid and/or synthesis of complementary DNA using non-complementary nucleic acids as primer and template (RNA- or DNA-templated DNA polymerase activity.

Provided are a separator for separating a nucleic acid amplification system, comprising a polyethylene wax, a solid paraffin wax, and a liquid paraffin wax. Also provided is a method for separating a nucleic acid amplification system, comprising using the separator as a separation layer to separate a nucleic acid amplification system within a same container. The separating layer can be broken by means of applying an external force, thereby mixing the nucleic acid amplification system.

Provided are a separator for separating a nucleic acid amplification system, comprising a polyethylene wax, a solid paraffin wax, and a liquid paraffin wax. Also provided is a method for separating a nucleic acid amplification system, comprising using the separator as a separation layer to separate a nucleic acid amplification system within a same container. The separating layer can be broken by means of applying an external force, thereby mixing the nucleic acid amplification system.

A method determines a number of copies of a DNA sequence that is present in a fluid. The method includes a division step, a setting up step, an identification step, and an evaluation step. In the division step, at least some of the fluid is divided into at least two compartments. In the setting up step, a reaction condition is set up for the fluid divided into the at least two compartments in order to allow a reaction in each of the at least two compartments and to obtain a reaction result in each case. In the identification step, a signal, for example an optical signal, is identified that represents the reaction results of the reactions that may have taken place in the compartments. In the evaluation step, the optical signal is evaluated in order to determine the number of copies.

The present invention relates to a method of detecting a genetic event, the method comprising steps of partitioning a sample from a subject into a plurality of partitions, and carrying out a digital polymerase chain reaction (dPCR) assay, to determine occurrence of said genetic event.

The present invention relates to a method of detecting a genetic event, the method comprising steps of partitioning a sample from a subject into a plurality of partitions, and carrying out a digital polymerase chain reaction (dPCR) assay, to determine occurrence of said genetic event.

Described is a nucleic acid ligand, a mixture thereof, and the use thereof. The mixture contains two or more nucleic acid polymerase substrate analogs. The nucleic acid polymerase substrate analog is a single nucleic acid molecule or nucleic acid molecule analog which forms complementary pairing within a molecule, or a single or two nucleic acid molecules or nucleic acid molecule analogs which form complementary pairing between molecules; and a structure formed thereby has the characteristics of a nucleic acid polymerase substrate. The nucleic acid polymerase substrate analog is suitable for all polymerases and can be widely used in the field of nucleic acid amplification. The 3′ end of the nucleic acid ligand has a modification which inhibits the extension thereof.

The present invention provides method essentially comprising the steps of (i) providing a DNA sample, (ii) providing at least a first pair of amplification primers which is capable of generating a first amplicon from a LINE sequence that has a size of less than 80 bp, (iii) providing at least a second pair of amplification primers which is capable of generating a second amplicon from a LINE sequence that has a size of more than 160 bp, (iv) performing a qPCR and determining cq values for each of the generated amplicons, and (v) determining the relative concentrations of said amplicons.

The present invention provides method essentially comprising the steps of (i) providing a DNA sample, (ii) providing at least a first pair of amplification primers which is capable of generating a first amplicon from a LINE sequence that has a size of less than 80 bp, (iii) providing at least a second pair of amplification primers which is capable of generating a second amplicon from a LINE sequence that has a size of more than 160 bp, (iv) performing a qPCR and determining cq values for each of the generated amplicons, and (v) determining the relative concentrations of said amplicons.