Among other things, the present invention is related to devices and methods of performing biological and chemical assays, particularly an easy sample manipulation and/or a rapid change or a rapid thermal cycling of a sample temperature is needed (e.g. Polymerase Chain Reaction (PCR) for amplifying nucleic acids).

Among other things, the present invention is related to devices and methods of performing biological and chemical assays, particularly an easy sample manipulation and/or a rapid change or a rapid thermal cycling of a sample temperature is needed (e.g. Polymerase Chain Reaction (PCR) for amplifying nucleic acids).

The present invention provides a method of quantification of a target nucleic acid, using at least any two of the genes SYT10, EPHA3, PLEKHF1 and KBTBD4 as control genes. In particular, the combination of the genes SYT10, EPHA3, PLEKHF1 and KBTBD4, known as the 4Plex, is provided as a control for nucleic acid quantification. The 4Plex has particular utility as a control for nucleic acid quantification by methylation-specific droplet digital PCR.

The present invention provides a method of quantification of a target nucleic acid, using at least any two of the genes SYT10, EPHA3, PLEKHF1 and KBTBD4 as control genes. In particular, the combination of the genes SYT10, EPHA3, PLEKHF1 and KBTBD4, known as the 4Plex, is provided as a control for nucleic acid quantification. The 4Plex has particular utility as a control for nucleic acid quantification by methylation-specific droplet digital PCR.

Provided herein are methods for labeling input DNA with oligonucleotide barcode sequences, and selective PCR amplification of DNA sequence variants across the targeted regions for variant quantitation.

Provided herein are methods for labeling input DNA with oligonucleotide barcode sequences, and selective PCR amplification of DNA sequence variants across the targeted regions for variant quantitation.

Provided is a kit for virus detection including a nucleic acid membrane containing a gold component and reacting with RNA polymerase to transcribe RNA, a biosensor for RNA polymerase detection based thereon, and RNA polymerase.

Provided is a kit for virus detection including a nucleic acid membrane containing a gold component and reacting with RNA polymerase to transcribe RNA, a biosensor for RNA polymerase detection based thereon, and RNA polymerase.

The invention includes, at minimum, an integrated, automated system for diagnosis of antibiotic resistance genes that performs microarray hybridization, washes, and reading, using a plurality of high-density glass and/or polymer microarray chips. The system may include one or more ring or circle-shaped microarray chips formed from glass and/or a polymer. The system may also include well plates pre-treated with one or more of fluorescently labeled primers, polymerase, dNTPs or other chemicals or reagents used to multiplex PCR identification of antibiotic resistance genes. The system may also include one or more of 96 or 128 sized well plates. The system may also include one or more centrifugation tubes having a nanopore filter fixed in place and/or a viscous solution for isolating smaller pathogens from larger host eukaryotic cells.

The invention includes, at minimum, an integrated, automated system for diagnosis of antibiotic resistance genes that performs microarray hybridization, washes, and reading, using a plurality of high-density glass and/or polymer microarray chips. The system may include one or more ring or circle-shaped microarray chips formed from glass and/or a polymer. The system may also include well plates pre-treated with one or more of fluorescently labeled primers, polymerase, dNTPs or other chemicals or reagents used to multiplex PCR identification of antibiotic resistance genes. The system may also include one or more of 96 or 128 sized well plates. The system may also include one or more centrifugation tubes having a nanopore filter fixed in place and/or a viscous solution for isolating smaller pathogens from larger host eukaryotic cells.