An apparatus for gene amplification includes a gene amplification chip including a well configured to accept a sample that is loaded into the well; the gene amplification chip being configured to: thermally dissolve the sample in the well so that a microbe present in the sample is thermally dissolved in the well to release genes in the microbe; and amplify the released genes in the well. The apparatus for gene amplification also includes a temperature controller configured to control a thermal dissolution temperature and a gene amplification temperature of the well.

An apparatus for gene amplification includes a gene amplification chip including a well configured to accept a sample that is loaded into the well; the gene amplification chip being configured to: thermally dissolve the sample in the well so that a microbe present in the sample is thermally dissolved in the well to release genes in the microbe; and amplify the released genes in the well. The apparatus for gene amplification also includes a temperature controller configured to control a thermal dissolution temperature and a gene amplification temperature of the well.

Described are oligonucleotides (LAMP primers), compositions, kits, and method for loop-mediated-isothermal amplification (LAMP) and/or detection of SARS-CoV-related betacoronaviruses. The methods can be used to in diagnosing SARS-CoV-2 infection.

Described are oligonucleotides (LAMP primers), compositions, kits, and method for loop-mediated-isothermal amplification (LAMP) and/or detection of SARS-CoV-related betacoronaviruses. The methods can be used to in diagnosing SARS-CoV-2 infection.

A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

Disclosed are methods for performing capillary electrophoresis on two or more nucleic acid samples. The methods employ a forward voltage to move a first sample forward from an inlet to an interrogation region in the capillary, then a backward voltage to move the first sample backward, and then a forward voltage again to move the first sample and a second sample forward. Systems and apparatuses for performing capillary electrophoresis are also provided.

Disclosed are methods for performing capillary electrophoresis on two or more nucleic acid samples. The methods employ a forward voltage to move a first sample forward from an inlet to an interrogation region in the capillary, then a backward voltage to move the first sample backward, and then a forward voltage again to move the first sample and a second sample forward. Systems and apparatuses for performing capillary electrophoresis are also provided.

The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.

The present invention relates to a detector for measuring fluorescence in a liquid sample and to devices for biochemical analyses comprising it, in particular to devices for performing analyses of real time PCR. The detector of the present invention has a series of advantages such as drastic simplification of the detection configuration, reduced costs, better performances due to the greater freedom in planning the optical configuration which allows dividing the detector itself into independent areas.