A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.

The disclosure provides a primer pair for identifying a gene segment of a male-specific molecular marker of American shad (Alosa sapidissima). The gene segment has a nucleic acid sequence represented by SEQ ID NO: 2, and the primer pair has nucleic acid sequences represented by SEQ ID NO: 3 and SEQ ID NO: 4.

The disclosure provides a primer pair for identifying a gene segment of a male-specific molecular marker of American shad (Alosa sapidissima). The gene segment has a nucleic acid sequence represented by SEQ ID NO: 2, and the primer pair has nucleic acid sequences represented by SEQ ID NO: 3 and SEQ ID NO: 4.

The disclosure relates to methods, compositions, and kits for the early determination of the sex of a fetus. The disclosure also provides methods, compositions, and kits for detecting fetal nucleic acids in biological samples (e.g., cell-free fetal DNA).

The disclosure relates to methods, compositions, and kits for the early determination of the sex of a fetus. The disclosure also provides methods, compositions, and kits for detecting fetal nucleic acids in biological samples (e.g., cell-free fetal DNA).

Systems and methods for validating that a DNA sample is from a test subject are disclosed. The test subject reports one or more characteristics (biological sex, ethnicity, and/or age) that may be predicted from the DNA sample. The predictions are compared to the reported characteristics to validate the DNA sample. To validate according to biological sex, the system determines a Y-chromosome signal based on counts of sequence reads for a gene specific to the Y chromosome and, similarly, an X-chromosome signal using another gene specific to the X chromosome. The biological sex is predicted based on a comparison of the two signals. To validate according to ethnicity, the system predicts ethnicity based on detected allele frequencies for SNPs specific to each chromosome. To validate according to age, the system calculates the methylation densities for age-informative CpG sites. The system utilizes trained regression models to predict the age using the methylation densities.

Systems and methods for validating that a DNA sample is from a test subject are disclosed. The test subject reports one or more characteristics (biological sex, ethnicity, and/or age) that may be predicted from the DNA sample. The predictions are compared to the reported characteristics to validate the DNA sample. To validate according to biological sex, the system determines a Y-chromosome signal based on counts of sequence reads for a gene specific to the Y chromosome and, similarly, an X-chromosome signal using another gene specific to the X chromosome. The biological sex is predicted based on a comparison of the two signals. To validate according to ethnicity, the system predicts ethnicity based on detected allele frequencies for SNPs specific to each chromosome. To validate according to age, the system calculates the methylation densities for age-informative CpG sites. The system utilizes trained regression models to predict the age using the methylation densities.

The present invention provides a molecular marker C42257 for rapidly identifying genetic sex of Marsupenaeus japonicus and applications thereof. The nucleotide sequence of the molecular marker C42257 is shown in SEQ ID NO:1. The nucleotide sequences of the primer pair of molecular marker C42257 used for detection are C42257F: GGGGGACAA ACAGAGACA (SEQ ID NO: 2) and C42257R: ATCGGGGTGGATTTAGAA (SEQ ID NO: 3), respectively. The molecular marker C42257 is not affected by the tissue specificity of M. japonicus and the environment, and the steps are simple and the results are obvious. The genomic DNA of M. japonicus and the primer pair are subjected to PCR reaction and electrophoresis detection. If a target band appears at 183 bp, it is a female M. japonicus. The molecular marker can also promote the establishment of the seedling breeding technology of all-female or high-female-ratio M. japonicus. Therefore, it has a broad application prospect.

A method for identifying the genetic sex of Portunus trituberculatus is provided. The SNP markers provided by the present invention presents biallelic heterozygosity in male individuals, but presents homozygous allele type in female individuals, and can be detected by PCR amplification and sequencing method, HRM method or KASP method. The detection method is flexible and the results are stable. Among them, the KASP and HRM detection methods have high throughput, fast detection speed, and simple application. It provides an important tool for the breeding of all-female population of P. trituberculatus.

A method for identifying the genetic sex of Portunus trituberculatus is provided. The SNP markers provided by the present invention presents biallelic heterozygosity in male individuals, but presents homozygous allele type in female individuals, and can be detected by PCR amplification and sequencing method, HRM method or KASP method. The detection method is flexible and the results are stable. Among them, the KASP and HRM detection methods have high throughput, fast detection speed, and simple application. It provides an important tool for the breeding of all-female population of P. trituberculatus.