The present invention aims at providing a specific antibody that can simply and rapidly detect Mycoplasma pneumoniae which is a causative bacterium of mycoplasma pneumonia, with high sensitivity, and also an immunological detection method and a kit containing the same antibody. The present invention makes it possible to diagnose infection with Mycoplasma pneumoniae more rapidly and specifically than the conventional method, by producing an antibody recognizing a specific epitope of P30 protein of Mycoplasma pneumoniae and performing an immunological detection using the antibody. Also, the present invention enables easy and rapid detection of Mycoplasma pneumoniae and diagnosis of infection with the same at a hospital or the like without need of specialized instruments or skilled techniques.

A highly sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect ZIKV nucleic acid in biological samples is described. The disclosed assay is capable of detecting as few as one RNA copy per μl and can be performed in a clinical or field setting with minimal equipment and technological expertise. Oligonucleotide primers and kits for detecting ZIKV nucleic acid are also described.

A highly sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect ZIKV nucleic acid in biological samples is described. The disclosed assay is capable of detecting as few as one RNA copy per μl and can be performed in a clinical or field setting with minimal equipment and technological expertise. Oligonucleotide primers and kits for detecting ZIKV nucleic acid are also described.

Provided herein are methods and compositions for providing identification and/or traceability of biological materials. In certain embodiments, methods are provided including steps of: determining a sequence of at least one unique identifier sequence in the genomic DNA of a biological entity; validating identification of the biological entity by verifying presence of the unique identifier sequence in the genomic DNA and comparing the sequence of the unique identifier sequence with a database to confirm uniqueness; providing an indication of acceptability to produce a biological material from the biological entity; and inputting the unique identifier sequence into a database entry of the database and associating the unique identifier sequence with identification and/or tracking information; thereby providing traceability by reading the unique identifier sequence and retrieving the corresponding database entry to obtain the identification and/or tracking information. Oligonucleotides, cassettes, and compositions for providing identification and/or traceability of biological materials are also provided.

Provided herein are methods and compositions for providing identification and/or traceability of biological materials. In certain embodiments, methods are provided including steps of: determining a sequence of at least one unique identifier sequence in the genomic DNA of a biological entity; validating identification of the biological entity by verifying presence of the unique identifier sequence in the genomic DNA and comparing the sequence of the unique identifier sequence with a database to confirm uniqueness; providing an indication of acceptability to produce a biological material from the biological entity; and inputting the unique identifier sequence into a database entry of the database and associating the unique identifier sequence with identification and/or tracking information; thereby providing traceability by reading the unique identifier sequence and retrieving the corresponding database entry to obtain the identification and/or tracking information. Oligonucleotides, cassettes, and compositions for providing identification and/or traceability of biological materials are also provided.

Provided is a method and a kit for detection of human papillomaviruses. Specifically, provided is a method for detecting at least 65 human papillomavirus genotypes. Also provided is a kit for detection of human papillomaviruses, which comprises one or more reagents capable of detecting at least 65 human papillomavirus genotypes.

Provided is a method and a kit for detection of human papillomaviruses. Specifically, provided is a method for detecting at least 65 human papillomavirus genotypes. Also provided is a kit for detection of human papillomaviruses, which comprises one or more reagents capable of detecting at least 65 human papillomavirus genotypes.

The invention provides compositions, devices, methods and kits allowing for rapid diagnosis of infectious diseases via extraction-free, direct PCR techniques using combined biological samples.

The invention provides compositions, devices, methods and kits allowing for rapid diagnosis of infectious diseases via extraction-free, direct PCR techniques using combined biological samples.

The present invention provides a means for accurately discriminating whether an onion is an onion with no pungent taste and/or tear-inducing property. The present invention relates to a method of discriminating traits of an onion, comprising a first determination step of determining presence of the nucleotide sequence of SEQ ID NO:1 corresponding to alliinase gene 1 in a nucleic acid derived from the onion, and a second determination step of determining presence of the nucleotide sequence of SEQ ID NO:2 corresponding to alliinase gene 2, wherein the onion is discriminated to be an onion with no pungent taste and/or tear-inducing property if the presence of the nucleotide sequence of SEQ ID NO:1 is not determined in the first determination step and the presence of the nucleotide sequence of SEQ ID NO:2 is determined in the second determination step.