The present invention provides a means for accurately discriminating whether an onion is an onion with no pungent taste and/or tear-inducing property. The present invention relates to a method of discriminating traits of an onion, comprising a first determination step of determining presence of the nucleotide sequence of SEQ ID NO:1 corresponding to alliinase gene 1 in a nucleic acid derived from the onion, and a second determination step of determining presence of the nucleotide sequence of SEQ ID NO:2 corresponding to alliinase gene 2, wherein the onion is discriminated to be an onion with no pungent taste and/or tear-inducing property if the presence of the nucleotide sequence of SEQ ID NO:1 is not determined in the first determination step and the presence of the nucleotide sequence of SEQ ID NO:2 is determined in the second determination step.

The present invention provides a method for testing the DNA of the source of the parasite in a sample, a method for diagnosing, using the testing method, whether a host object is suffered with a parasitic infection, a method for determining a treatment effect on a parasitic infection, and a method for screening a candidate treatment of a parasitic infection. Also provided is a kit for the methods.

The present invention provides a method for testing the DNA of the source of the parasite in a sample, a method for diagnosing, using the testing method, whether a host object is suffered with a parasitic infection, a method for determining a treatment effect on a parasitic infection, and a method for screening a candidate treatment of a parasitic infection. Also provided is a kit for the methods.

The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

The present invention refers to a device comprising polytetrafluorethylene (PTFE) filters and the use of the same for collecting, detecting and identifying organisms present in air ecosystems. The invention also provides a method suitable for the capture, detection and identification of whole airborne biological particles, including viruses and other important air pathogens, which involves the use of the device. This method allows toperform organism, preferably viral, metagenomics to sequence all DNA and RNA organisms captured in the filters. This methodology may be used to detect, for instance, SARS-CoV2 particles in air samples.

Provided is a kit for virus detection including a nucleic acid membrane containing a gold component and reacting with RNA polymerase to transcribe RNA, a biosensor for RNA polymerase detection based thereon, and RNA polymerase.

Provided is a kit for virus detection including a nucleic acid membrane containing a gold component and reacting with RNA polymerase to transcribe RNA, a biosensor for RNA polymerase detection based thereon, and RNA polymerase.

Compositions and methods are provided for creating and promoting biallelic targeted modifications to genomes within cells and for producing non-human animals comprising the modified genomes. Also provided are compositions and methods for modifying a genome within a cell that is heterozygous for an allele to become homozygous for that allele. The methods make use of Cas proteins and two or more guide RNAs that target different locations within the same genomic target locus. Also provided are methods of identifying cells with modified genomes.

Compositions and methods are provided for creating and promoting biallelic targeted modifications to genomes within cells and for producing non-human animals comprising the modified genomes. Also provided are compositions and methods for modifying a genome within a cell that is heterozygous for an allele to become homozygous for that allele. The methods make use of Cas proteins and two or more guide RNAs that target different locations within the same genomic target locus. Also provided are methods of identifying cells with modified genomes.