The present invention relates to nucleic acid constructs comprising a first polynucleotide encoding a signal peptide from a bacterial alpha-amylase a second polynucleotide encoding a polypeptide having hexosaminidase activity; expression vectors and host cells comprising said nucleic acid constructs; and methods for producing polypeptides having hexosaminidase activity.

The present invention relates to a bacterial host cell in which a first chromosomal gene encoding a first alanine racemase and a second chromosomal gene encoding a second alanine racemase have been inactivated. Said bacterial host cell comprises – either on a plasmid comprising at least one autonomous replication sequence or present as multiple copies in the chromosome – a gene expression cassette comprising a polynucleotide encoding at least one polypeptide of interest, operably linked to a promoter, and a polynucleotide encoding a third alanine racemase, operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

The present disclosure is generally related to compositions and methods for constructing and/or obtaining B. licheniformis cells (e.g., protein production hosts) comprising enhanced protein production capabilities. Thus, certain embodiments are related to genetically modified Bacillus licheniformis strains derived from parental B. licheniformis strains producing increased amounts of one or more proteins of interest.

The present invention relates to an animal feed or an animal feed additive comprising Bacillus strains which improve the health and performance of production animals, and the use of such.

The present invention relates to a method for recovering a protein of interest from a fermentation broth by adding a salt of a divalent cation and increasing the pH.

Disclosed is a method for synthesizing a diglyceride. The diglyceride is obtained by mixing a fatty acid donor with glycerol, partial glyceride lipase, and monoglyceride lipase by adding water, then subjecting the same to an esterification reaction, with a reaction time of 8 to 24 hours, and further separating and purifying the same. In the present invention, monoglyceride lipase is used to promote the reaction efficiency of partial glyceride lipase in the esterification reaction, so as to increase the synthesis rate of diglyceride. Compared with a single enzyme, the synthesis time is shortened by half or more, and 45.50% or more of diglyceride is obtained after the esterification reaction. Since substantially no triglyceride is generated in products, the content of DAG reaches 98% or more after the same has been purified by means of molecular distillation.

The present disclosure is generally related to compositions and methods for constructing and obtaining Bacillus licheniformis cells having increased protein production phenotypes. Thus, certain embodiments are related to modified B. licheniformis cells derived from parental B. licheniformis cells. Certain embodiments are related to modified B. licheniformis cells comprising a modified rghR locus. Certain embodiments are related to modified B. licheniformis cells having a modified rghR locus and comprising an increased protein productivity phenotype. In certain other embodiments, modified B. licheniformis cells having a modified rghR locus produce a reduced amount of red pigment. In certain other embodiments, modified B. licheniformis cells comprise an increased protein productivity phenotype and produce a reduced amount of red pigment.

The invention relates to compositions comprising microbial strains and methods for use of the compositions in removing pollutants in waste, water, or soil, such as remediating dye and lignin, reducing contaminants, degrading paper (such as flushable or disposable or non-flushable wipes), reducing odor, reducing chemical oxygen demand (COD), and combinations thereof, in the water, the soil, or the waste or for use in administration to animals in the feed or drinking water of the animals. More particularly, the invention relates to compositions of isolated Bacillus strains selected from the group consisting of isolated Bacillus strains Bacillus licheniformis MDG-1000 (NRRL No. B-67888), Bacillus licheniformis MDG-1001 (NRRL No. B-67889), Bacillus subtilis/amyloliquefaciens MDG-8001 (NRRL No. B-67890), Bacillus pumilus MDG-1047 (NRRL No. B-67891), Bacillus amyloliquefaciens MDG1607 (NRRL No. B-67666), Bacillus subtilis MDGV18 (NRRL No. B-67665), Bacillus pumilus MDGV17 (NRRL No. B-67664), Bacillus subtilis MDG-1728 (NRRL No. B-67618), Bacillus megaterium MDG-2705 (NRRL No. B-67619), and strains having all of the identifying characteristics of these strains, and combinations thereof, and methods for use of these strains for removing pollutants in waste, water, or soil, such as remediating dye and lignin, reducing contaminants, degrading paper (such as flushable or disposable or non-flushable wipes), reducing odor, reducing chemical oxygen demand (COD), and combinations thereof, in the water, the soil, or the waste or for use in administration to animals in the feed or drinking water of the animals.

A method for transforming organic waste into carbon using sequential physical and biological degradation, including fermentation, drying under vacuum and elevated temperature followed by heating to a temperature of between 300° C. and 500° C. to promote carbonization and production of charcoal.

A method of using lactase for generating galactooligosaccharide as well as the preparation and an application of the lactase are provided. Lactase (BglD305 derived from Bacillus circulans B2301 and BglD derived from Bacillus circulans ATCC 31382) molecules from two sources are taken as the basis for molecular evolution, so as to obtain new lactase enzyme molecules with high galactooligosaccharide synthesis efficiency and good expression performance. The high-producing strain lactase is further constructed, the lactase can be efficiently synthesized during the submerged fermentation, and the enzyme molecule is secreted into the culture medium, the high-activity enzyme preparation is directly prepared from the fermentation supernatant, and the lactase expression level can achieve 2208 U/mL. As the result, the fermentation manufacturing cost of lactase is reduced, the fermentation manufacturing process is simplified, and the quality of the lactase preparation is improved.