The present invention relates to novel subspecies of Bacillus. The novel subspecies can improve health and performance of production animals. In one embodiment the Bacillus subtilis subspecies has activity against Clostridium perfringens and/or E. coli. The invention further relates to compositions comprising one or more strains of the Bacillus subtilis subspecies and to use of the strain(s) of the Bacillus subtilis subspecies in an animal feed.

The present invention provides a recombinant Bacillus subtilis JY011802 that can produce sublancin in a high yield, which was deposited at the China General Microbiological Culture Collection Center on Oct. 31, 2018 with an accession number of CGMCC No. 16667, and an application thereof. The yield of the sublancin produced by the recombinant Bacillus subtilis can reach 3100 mg/L.

The invention relates to a water-soluble fermented pea extract. The invention also relates to a process for the preparation thereof and to the use thereof in the human and animal nutrition industry as well as in the pharmaceutical, nutraceutical and cosmetics industries.

The invention relates to treatment of a plant with one or more microorganisms for inhibiting a plant pathogen. More particularly, the invention relates to isolated Bacillus strains, and strains having all of the identifying characteristics of these strains, and combinations thereof, for a use comprising the above-mentioned use.

Provided are biologically pure bacterial isolates characterized by a genome structure at least 90% similar to a genome structure of a bacterial species selected from the group consisting of: Streptomyces sp. E128 having an NRRL Accession No. B-67462, Bacillus amyloliquefaciens A190 having an NRRL Accession No. B-67464, Bacillus subtilis P243 having an NRRL Accession No. B-67459, Bacillus thuringiensis M979 having an NRRL Accession No. B-67457, Massilia aurea P63 having an NRRL Accession No. B-67461, Rhodococcus sp. G706, Stenotrophomonas maltophilia E132 having an NRRL Accession No. B-67460, Streptomyces aurantiacus A918, Streptomyces badius 0180, Streptomyces mirabilis B670 having an NRRL Accession No. B67463, Streptomyces scopuliridis F427 having an NRRL Accession No. B-67458, and Streptomyces sp. L219. Also provided are whole cell broth or lysates thereof, and polynucleotide, polypeptides and constructs expressing same, compositions-of-matter comprising same and methods using same for killing or inhibiting the development of insects.

Disclosed are strains capable of reducing heavy metal contents in vegetables and improving quality of vegetables and application thereof. Specifically, the strains are strains W7 and W25, which can reduce heavy metal contents, especially cadmium (Cd) contents, in plants, promote growth of plants, increase Vc and soluble protein contents in plants, and increase a microbial abundance and a urease activity in the soil at the same time, and can be widely applied to the fields of soil improvement, crop biomass increase, and soil heavy metal restoration.

Provided is a method for producing a heavy chain variable domain of a heavy-chain antibody (VHH) with a high yield. The method for producing a VHH includes culturing a Bacillus bacterium into which a gene that encodes variable domain of heavy chain of heavy-chain antibody is introduced and which is deficient in an extracellular protease.

Provided are biologically pure bacterial isolates characterized by a genome structure at least 90% similar to a genome structure of a bacterial species selected from the group consisting of: Streptomyces sp. E128 having an NRRL Accession No. B-67462, Bacillus amyloliquefaciens A190 having an NRRL Accession No. B-67464, Bacillus subtilis P243 having an NRRL Accession No. B-67459, Bacillus thuringiensis M979 having an NRRL Accession No. B-67457, Massilia aurea P63 having an NRRL Accession No. B-67461, Rhodococcus sp. G706, Stenotrophomonas maltophilia E132 having an NRRL Accession No. B-67460, Streptomyces aurantiacus A918, Streptomyces badius O180, Streptomyces mirabilis B670 having an NRRL Accession No. B67463, Streptomyces scopuliridis F427 having an NRRL Accession No. B-67458, and Streptomyces sp. L219. Also provided are whole cell broth or lysates thereof, and polynucleotide, polypeptides and constructs expressing same, compositions-of-matter comprising same and methods using same for killing or inhibiting the development of insects.

A genetically engineered strain having high-yield of L-valine is disclosed. Starting from Escherichia coli W3110, an acetolactate synthase gene alsS of Bacillus subtilis is inserted into a genome thereof and overexpressed; a ppGpp 3′-pyrophosphate hydrolase mutant R290E/K292D gene spoTM of Escherichia coli is inserted into the genome and overexpressed; a lactate dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB, and genes frdA, frdB, frdC, frdD of four subunits of fumaric acid reductase are deleted from the genome; a leucine dehydrogenase gene bcd of Bacillus subtilis replaces a branched chain amino acid transaminase gene ilvE of Escherichia coli; and an acetohydroxy acid isomeroreductase mutant L67E/R68F/K75E gene ilvCM replaces the native acetohydroxy acid isomeroreductase gene ilvC of Escherichia coli. Furthermore, the L-valine fermentation method is improved by using a two-stage dissolved oxygen control. The L-valine titer and the sugar-acid conversion rate are increased.

An improved bacterial oil treatment composition or pool for handling a decommissioned oil cable, which may be laid in particular as part of a power grid in the ground. The invention further relates to a bacteria growth culture medium containing the bacterial oil treatment composition for refurbishing an oil cable and a corresponding use.