A method of producing a composition of Gram-negative bacteria includes (a) subjecting a wet mass of Gram-negative bacteria to spray drying; and (b) contacting the spray-dried Gram-negative bacteria from step (a) to a mixture including at least one hydrophobic, partially water-insoluble polyglycerol ester in combination with at least one emulsifier to form the composition of Gram-negative bacteria, wherein the polyglycerol ester has a general formula (I) of,

M.sub.aD.sub.bT.sub.cFormula (I) wherein, M=[C.sub.3H.sub.5(OR).sub.2O.sub.1/2], D=[C.sub.3H.sub.5(OR).sub.1O.sub.2/2], T=[C.sub.3H.sub.5O.sub.3/2], a=1 to 10; b=0 to 10; c=0 to 3;
wherein, the sum total of a+b+c is 1 to 20,
wherein the radicals R are independently selected from acyl radicals RC(=0)- and H, with the proviso that at least one radical R is not equal to H;
wherein the radicals R are independently selected from monovalent aliphatic, saturated or unsaturated hydrocarbon radicals with 3 to 39 carbon atoms.

The present invention relates to a method of producing a composition of Gram-negative bacteria, the method comprising (a) subjecting a wet mass of Gram-negative bacteria to spray drying; and (b) contacting the spray-dried Gram-negative bacteria from step (a) to a compound, wherein the compound has a general formula (I) of,

R.sup.1O[(C.sub.2H.sub.3R.sup.2)O].sub.nHFormula (I) where R.sup.1 is a monovalent aliphatic radical having 1 to 22, preferably 2 to 10, especially 3 to 4, carbon atoms; R.sup.2 is in each case independently a hydrogen radical or a methyl radical; and n is a number from 1 to 300, preferably from 5 to 100, especially from 10 to 30, with the proviso that at least one R.sup.2 radical is a methyl radical.

Customized recombinant proteins are designed and produced by cultures of Pseudomonas bacteria, including natural and recombinant silk proteins, fluorescent proteins, and elastin-like proteins (ELPs). The recombinant genes can be expressed via insertion directly into the Pseudomonas bacteria, or via the transformation of a suitably designed recombinant plasmid. Advantageously, the carbon source used as the nutrient source by the Pseudomonas bacteria is derived from non-traditional nutrient sources, such as exogenous rhamnolipids, hydrocarbons, polyolefins, polyesters, and pyrolysis products of waste plastic, e.g., pyrolysis products of polyethylene or poly (ethylene terephthalate). The waste feedstocks can be added to particularly designed growth media for sustained bacterial culture and protein production. These feedstocks allow for upcycling of plastic waste into high value protein products, such as recombinant silk fibroins.

Provided are a lipase mutant and an application thereof. Specifically, one or more mutations selected from A262H, A338V, V3641, A158PN, and 1159N are generated on the basis of an amino acid sequence as shown in SEQ ID NO: 1; and compared with a parental lipase, a change in the structure and function of a protein occurs in the lipase mutant, and the stereoselectivity is improved, such that a usage amount of an enzyme is decreased to a certain extent, the post-treatment difficulty is reduced, and the lipase mutant is suitable for industrial production.

A method to reduce concentration of a VOC (volatile organic compound) in air in contact with a coated surface comprising: providing a surfaceencapsulation of whole microorganism of Pseudomonas putida in a sol-gel matrixapplication of the obtained sol-gel matrix to the surface to form the coated surfaceexposing the air to the coated surface.

A biological product for the purification of water, soil, industrial wastewater from chemicals that are resistant to degradation, such as pesticides and oil, contains a composition of strains of bacteria Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999, and Rhodococcus erythropolis VKPM Ac-1882 in a weight ratio of (1-2);(1-2):1. The biological product can further contain sorbent, organic and mineral supplements, and stimulating agents. The biological product can be used for the decomposition of degradation-resistant pesticides and oil and has plant growth stimulating and fungicidal activity.

Disclosed herein are methods, compositions and systems useful for genetically engineering subcellular compartments such as OMVs for synthetic biology applications. In an embodiment, genetically engineered bacteria use OMVs to secrete compounds or proteins of interest extracellularly where the compounds or proteins of interest can be isolated from the growth media.

The present disclosure uses a combination of transcriptomics and genetics to demonstrate that P. putida PhaG is likely a 3-hydroxyacyl-ACP thiolase rather than a 3-hydroxyacyl-ACP:CoA transacylase. Deletion of two 3-hydroxyacyl CoA synthases results in the abolishment of PHAs as a product and leads to the accumulation of free medium chain length 3-hydroxyacyl acids as products into the culture supernatant under nitrogen starvation conditions. The present disclosure demonstrates a biological route to the production of 3-hydroxyacyl acids for use as industrial chemicals.

The present invention is in the field of genetically modified enzyme and microorganism comprising such a modified enzyme for the production of raspberry ketone or zingerone. The modified enzyme is a polyketide synthase (PKS) issued or derived from a wild type PKS. The modified PKS has the capability to produce vanillylidene acetone from feruloyl-CoA and/or 4-hydroxybenzalacetone from 4-coumaroyl-CoA in a more effective way as compared to wild type BAS or wild type PmPKS, in particular in recombinant bacteria strains.

Leaf-branch compost cutinase (LCC) and other cutinase mutants with activity over ranges of temperature and pH are provided.