The present disclosure provides recombinant Staphylococcus bacterium (e.g. S. epidermidis) that are dependent on D-alanine for growth. In one aspect, the disclosure features a recombinant Staphylococcus bacterium comprising two inactivated alanine racemase genes (?alr1?alr2); and an inactivated D-alanine aminotransferase (dat) gene. In another aspect, the disclosure features a method of making the recombinant Staphylococcus bacterium.

Disclosed is a method for identifying causative bacteria of sepsis, the method comprising: a step of performing a PCR method using a sample collected from a subject and a combination of sets of primers each specific to the full-length or a partial region of each of gyrB gene of Escherichia coli, nuc gene of Staphylococcus aureus, atlE gene of Staphylococcus epidermidis, gyrB gene of Klebsiella pneumoniae and rpoB gene of Enterococcus spp.; and a step of detecting the presence or absence of amplification of the full-length or the partial region of each of the genes by using a combination of probes each specific to DNA of the full-length or the partial region.

The present disclosure provides a bacterium-contained hydrogel including: hydrogel, bacteria, and metabolites. The hydrogel includes a plurality of polymer chains crosslinked with each other, and each of the polymer chains include at least one polyethylene glycol chain. The bacteria are located in the hydrogel, and the bacteria use the polymer chains as a carbon source for fermentation. The metabolites are produced from the fermentation of the bacteria using the polymer chains as a carbon source. The metabolites distribute in the hydrogel. The present disclosure also provides a method for making bacterium-contained hydrogel.

The present disclosure provides recombinant Staphylococcus bacterium (e.g. S. epidermidis) that are dependent on D-alanine for growth. In one aspect, the disclosure features a recombinant Staphylococcus bacterium comprising two inactivated alanine racemase genes (alr1alr2); and an inactivated D-alanine aminotransferase (dat) gene. In another aspect, the disclosure features a method of making the recombinant Staphylococcus bacterium.

The antimicrobial effect of extracellular metabolite composition from Bacillus coagulans MTCC 5856 is described.

The antimicrobial effect of extracellular metabolite composition from Bacillus coagulans MTCC 5856 and composition comprising from about 61% w/w of thymol, about 38% of monolaurin and about 1% w/w of magnolol obtained from supercritical fluid extracts of Magnolia officinalis is described.

Process of inhibiting microbial biofilm formation using extracellular metabolite composition from Bacillus coagulans MTCC 5856 and composition comprising from about 61% w/w of thymol, about 38% w/w of monolaurin and about 1% w/w of magnolol obtained from supercritical fluid extracts of Magnolia officinalis is described.

Method of extracting a partially purified extracellular metabolite composition from Bacillus coagulans MTCC 5856 and its antimicrobial effects thereof are described.

The present invention relates to media compositions for culturing bacteria. In particular, the present invention relates to media for culture and/or fermentation of Clostridia bacteria.

The present invention relates to a method of treating organic waste comprising the steps of: treating the organic waste with a microorganism composition comprising the following microorganism species: Niallia sp., Stenotrophomonas sp. and Staphylococcus sp., wherein the treatment step is conducted under aerobic environment with stirring to convert the organic waste to an organic fertilizer.