A manufacturing method of precision machine tool bearing with high precision stability includes the procedures: (1) microstructural stabilization of bearing body: by cold ring rolling, two liquid quenching, ultrasonic assisted multiple cryo-tempering treatment and stress ageing treatment, the bearing body with high microstructure stability can be obtained; (2) precision machining; (3) internal stress relaxation of bearing body: after precision machining, by executing magnetic treatment on the bearing body, bearing ring with high microstructure stability and low internal stresses can be obtained; and (4) bearing assembly: finally precision machine tool bearing with high precision stability can be obtained. Considering that the critical factors affecting the precision stability of bearing is the degree of microstructure stability and internal stresses, by improving the microstructure stability and reducing residual stress in multistage manufacture phase, precision stability of precision machine tool bearing should be promoted.