A microfluidic chip is provided for self-sorting highly motile, morphologically normal sperm cell with high DNA integrity from a fresh semen sample. The sperm self-sorting microfluidic chip has one or more inlet chambers, and sperm collection outlet chamber(s), and the middle of the channel features various micro-fabricated structures in different geometrical shapes and orientations, with varying periodicities and patterns, such as an array of micro-fabricated pillars that facilitate the transport of the active and healthy sperm into the outlet chamber.

This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.

This disclosure concerns a cytometry system including a handling system that enables presentation of single cells to at least one laser source. The laser source is configured to deliver light to a cell within the cells in order to induce bond vibrations in the cellular DNA. The system further includes a detection facility that detects the signature of the bond vibrations, wherein the bond vibration signature is used to determine the folding or packing of the DNA.

A device for selecting sperm includes a stack of a plurality of layers of a material. The stack has an inlet end and an outlet end. Each layer of the material includes a plurality of sperm selection microchannels. Each sperm selection microchannel has a respective microchannel inlet at the inlet end of the stack and extends to a respective microchannel outlet at the outlet end of the stack.

The invention provides methods of flow-cytometrically processing sperm cells in which greater than 25% of sperm cells in the population to be processed have abnormal morphology.

A method of distinguishing between a first type of object and a second type of object in a fluid column by receiving output light from an objects in the fluid column, generating an electrical signal in response to the output light, and compensating for position dependence of the object in the fluid column.

The present invention relates to a biosensor and applications thereof for the quantification of sperm function. Methods and tools for diagnosis of male infertility are also disclosed herein.

A motility-based spermatozoa separation device has a hollow cylindrical body that includes an internal passageway. The hollow cylindrical body is configured for insertion into a test tube having a semen sample placed therein. A sperm separating member is disposed in the internal passageway. When the hollow cylindrical body is inserted into the test tube, the sperm separating member divides the test tube into an upper cavity and a lower cavity. A suitable media can be inserted in the internal passageway (e.g., the media flowing downwardly through the sperm separating member and onto the semen sample located in the lower cavity) placing the lower cavity in fluid communication with the upper cavity. Progressively motile spermatozoa from the semen sample can swim from the lower cavity toward the sperm separating member, swimming upwardly therethrough, and into the upper cavity to be obtained therefrom.

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

A cryopreserved dairy goat semen metabolite screening and diluent preparation method: preserve semen with base diluent until the day 5, select samples on the days 0, 1, 3 and 5 for metabolome sequencing, screen out gamma-aminobutyric acids decreasing significantly with the preservation time, add different concentrations of gamma-aminobutyric acids to detect sperm viability and motility, and determine the optimal concentration of gamma-aminobutyric acids. The optimal addition concentration of gamma-aminobutyric acids is 1.0 g/L, ensuring that the viability and motility of sperms preserved until the day 5 reach 56.49% and 53.69% and that plasma membrane integrity and acrosome integrity reach 58.93% and 60.12%, and effectively improving the oxidation resistance of sperms and reduce oxidative damage. The invention effectively improves the semen cryopreservation effect, has important significance for reducing breeding stock and improving artificial insemination and reproductive efficiencies in the animal husbandry, high popularization and application prospects and economic values.