The present invention relates to transgenic microalgae for the production of cell wall degradative enzymes having a heat-stable cellulolytic activity (HCWDEs) and their relative uses in the biodegradation of cellulose or lignocellulose sources in the industrial field.

A process for site-specific immobilization of an enzyme on a polymer involving pairing of an enzyme and polymer to optimize cross-linking between the enzyme and the polymer while avoiding conformational or biochemical inhibition of enzyme activity. The process involves site specific interaction in a multiple phase synthesis within a buffered reaction medium and in an inert atmosphere. The reaction kinetics of the conjugation are modulated by chilling the reaction medium containing the enzyme and polymer to about the maximum solvent density of the reaction medium. The cross-linking of the enzyme and a polymer pair performed produces an enzymatically active, stable conjugate.

The instant invention relates to a filamentous fungal host cell producing a polypeptide of interest, said host cell comprising one or more native or heterologous polynucleotide encoding a long non-coding RNA (lncRNA), wherein said lncRNA comprises more than 262 nucleotides and comprises a contiguous nucleotide sequence at its 3′ end, absent any poly-adenylation tail, that is at least 70% identical to that of SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 19, SEQ ID NO:20 or SEQ ID NO:21; and/or said lncRNA comprises 2 or more Xyr1 binding sequences, as well as methods of producing a polypeptide of interest in said host cells and methods of improving the production of a polypeptide of interest.

The disclosure provides recombinant LAC polynucleotides encoding a laccase (LAC) polypeptide capable of polymerizing caffeyl alcohol, vectors and cells including the recombinant LAC polynucleotide. The disclosure also provides transgenic plants including cells having the recombinant LAC polynucleotide of the present disclosure and methods of increasing production of C-lignin in a plant.

The technical field of enzyme immobilization, and particularly, an organic-inorganic hybrid nanoflower and a preparation method thereof. The organic-inorganic hybrid nanoflower is a flower-like immobilized enzyme formed by self-assembly of a layered rare earth compound as an inorganic carrier and a biological enzyme as an organic component. The layered rare earth compound is Ln.sub.2(OH).sub.5NO.sub.3.Math.nH.sub.2O, where Ln is one or more of La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, or Y, and n=1.1-2.5. The biological enzyme is one or more of α-amylase, horseradish peroxidase, or laccase. A layered rare earth compound is used as the inorganic carrier for the organic biological enzyme to form the flower-like immobilized enzyme. The immobilized enzyme has better stability and higher catalytic performance when compared with a free enzyme.

The purpose of the present invention is to provide a processing technology for enhancing the crosslinkage effect of a protein. A processed protein obtained by a method of producing a processed protein, said method comprising a crosslinkage step for treating a protein with laccase and transglutaminase, has an enhanced crosslinkage effect.

The purpose of the present invention is to provide a novel feature by which the strength of tofu can be increased with a method for producing tofu using an enzyme. Tofu obtained by a tofu production method that includes a crosslinking step in which laccase is acted on soy milk exhibits increased strength, and when the tofu production method thereof further includes a boiling step after the crosslinking step, the strength of the obtained tofu is dramatically improved.

The present invention provides methods and compositions for increasing lignin degradation to produce a biological product. Also provided are methods for increasing expression of laccase in a bacterial species to produce increased lignin degradation. Also provided are bacterial cells and commodities or commodity produces produced from such methods.

The current invention discloses enzyme-based compositions for converting raw natural fibres from plant derived biomass into high quality textile grade fibres. The invention discloses at least one multi-component enzymatic formulation, and the optimal conditions for using these enzymatic formulations, which result in production of textile grade fibres from raw natural fibres. These textile grade fibres can be used in any industry, because of their high-quality parameters, and high spinnability index.

The present invention relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the invention relates to a polypeptide with laccase activity comprising an amino acid sequence that is at least 90% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises at least one amino acid selected from the group consisting of Alanine, Proline, Aspartic acid, Isoleucine, Proline, Proline, Threonine and Proline at a position corresponding to of positions 253, 128, 384, 364, 292, 450, 33 and 322 in SEQ ID NO: 1 respectively.