The disclosure provides systems, methods, reagents, apparatuses, vectors, and host cells for the discovery and evolution of metabolic pathways that produce small molecules that modulate enzyme function.

Provided is a method for screening a Corynebacterium constitutive expression vector promoter on the basis of transcriptome sequencing; and further provided are the Corynebacterium constitutive expression vector promoter screened on the basis of transcriptome sequencing, an expression vector comprising the promoter, a recombination strain obtained by transforming a host cell Corynebacterium glutamicum using the expression vector, and applications thereof.

Provided are engineered transposable elements, gene transfer systems comprising the engineered transposable elements, as well as methods and kits for using the same. The compositions, systems, and methods are useful for inserting a heterologous nucleic acid into a target nucleic acid in vitro or in a cell.

This disclosure relates to methods of quantifying affinity and selectivity of compounds for target proteins in living cells.

Compounds, composition, and kits are provided for use in methods for the assisted folding of protein fragments of a of choice point larger protein by means of induced proximity, forced by specific nucleic acid hybridizations between a target nucleic acid molecule and complementary nucleic acid molecules appended to the protein fragments of interest.

The present disclosure provides a technique for designing an epitope region-bridging biparatopic antibody, and also provides, through this technique, an antibody that has an improved agonist or antagonist function for an antigen or that promotes or enhances a chemical reaction catalyzed by an enzyme serving as an antigen. Provided is an epitope region-bridging biparatopic antibody, an antigen-binding fragment thereof or a functional equivalent of the same that has antigen specificities to a first epitope belonging to a first epitope group, among a plurality of epitope region groups in an antigen, and to a second epitope belonging to a second epitope group contained in an epitope region different from the epitope region in which the first epitope group is contained, said antibody comprising the sequences of a heavy chain variable region and a light chain variable region derived from an antibody against the first epitope and the sequences of a heavy chain variable region and a light chain variable region derived from an antibody against the second epitope.

The present invention relates to a dual cassette reporter system capable of assessing target gene translation and target gene product folding. The present invention further relates to vectors and host cells comprising the dual cassette reporter system. In addition the invention relates to the use of the dual cassette reporter system for assessing target gene translation and target gene product folding.

Provided herein is a 3-dimensional lattice microarray system for DNA sequence detection and analysis. The system has a plurality of bifunctional polymer linkers, on one end of which are attached nucleic acid probes where each have a sequence complementary to signature nucleotide sequences in pathogens, plants or animals. The other end of the bifunctional polymer linker is attached to a solid support by non-covalent or covalent means. Each of the nucleic acid probes have terminal thymidine bases at the 5′ and 3′ ends that permit attachment of the probes to the bifunctional polymer linkers. Also provided is a customizable microarray kit is provided that contains the solid support, linkers, probes, solvent mixture and instructions to use the kit.

Provided are: a polymeric IgA-type recombinant antibody; a medicine containing this polymeric IgA-type recombinant antibody as an active ingredient; a method for producing this polymeric IgA type antibody, the method including the step of coexpressing an IgA-type antibody heavy-chain protein, an antibody light-chain protein, an antibody J-chain protein, and a secretory component protein within a single cell; and a method for improving the antigen-binding activity or neutralizing activity of this antibody, the method including the step of making an antibody into a polymeric IgA-type.

Disclosed herein are novel compositions and methods for elucidating biological activity and detection of molecular function. The methods and compositions disclosed herein can comprise the use of one or more minimotifs and a minimotif database for integrating and coordinating orthogonal knowledge derived from a variety of technological endeavors to provide systemic models representing complex biological and molecular interactions ranging from individual cells to entire organisms. The methods and compositions disclosed herein can utilize information related to biometrics including protein/protein interaction, and gene/gene interaction for evaluating cellular functions and cellular mechanisms.