The present disclosure relates to a transformant for producing 2,5-furandicarboxylic acid. The transformant for producing 2,5-furandicarboxylic acid includes a Pseudomonas putida and at least one exogenous gene. The exogenous gene is an HmfH gene or an HMFO gene, and the exogenous gene is integrated into the chromosome of the Pseudomonas putida.

The invention relates to vectors and methods for de-repressing Cas systems in host cells.

The invention relates to vectors and methods for de-repressing Cas systems in host cells.

The invention relates to vectors and methods for de-repressing Cas systems in host cells.

The disclosure discloses recombinant Pseudomonas plecoglossicida for producing L-xylose and application thereof, and belongs to the technical field of bioengineering. According to the disclosure, a synthesized 2-ketogluconate reductase gene and a 2,5-diketogluconate reductase gene derived from Corynebaterium ATCC 31090 and a pyruvate decarboxylase gene derived from Saccharomyces cerevisiae are successfully expressed in a host P. plecoglossicida by a double plasmid system, and an obtained genetically engineered strain is fermented for 56 h in a shake flask, where the yield of L-xylose reaches 16.2 g/L, and the transformation rate reaches 20.3%; the obtained genetically engineered strain is fermented for 48 h and 44 h in 3 L and 15 L fermentors, respectively, where the yields of L-xylose reach 37.6 g/L and 45.8 g/L, respectively, and the glucose transformation rates are 47.0% and 57.3%, respectively. The method has the advantages of low raw material cost, no pollution to the environment, simple operation, and important economic and social benefits.

The disclosure discloses recombinant Pseudomonas plecoglossicida for producing L-xylose and application thereof, and belongs to the technical field of bioengineering. According to the disclosure, a synthesized 2-ketogluconate reductase gene and a 2,5-diketogluconate reductase gene derived from Corynebaterium ATCC 31090 and a pyruvate decarboxylase gene derived from Saccharomyces cerevisiae are successfully expressed in a host P. plecoglossicida by a double plasmid system, and an obtained genetically engineered strain is fermented for 56 h in a shake flask, where the yield of L-xylose reaches 16.2 g/L, and the transformation rate reaches 20.3%; the obtained genetically engineered strain is fermented for 48 h and 44 h in 3 L and 15 L fermentors, respectively, where the yields of L-xylose reach 37.6 g/L and 45.8 g/L, respectively, and the glucose transformation rates are 47.0% and 57.3%, respectively. The method has the advantages of low raw material cost, no pollution to the environment, simple operation, and important economic and social benefits.

A generic type I CRISPR-Cas-based genome-editing system that can be used in microbial hosts having diverse genetic backgrounds has been established. The chromosomal integration system overcomes the limitations of narrow host range and the requirement for antibiotics to maintain propagation and expression which are associated with plasmid-encoded Cas proteins. Compositions and methods for a chromosomal integrated type I-F CRISPR-Cas system for programmable genome editing and robust gene regulation are provided. In some embodiments, the compositions and methods are effective to selectively and specifically edit and/or regulate the genome of multiple microbial species with diverse genotypes. Compositions and methods for gene editing and/or gene regulation in Pseudomonas spp, such as multiple strains of P. aeruginosa, are described.

A generic type I CRISPR-Cas-based genome-editing system that can be used in microbial hosts having diverse genetic backgrounds has been established. The chromosomal integration system overcomes the limitations of narrow host range and the requirement for antibiotics to maintain propagation and expression which are associated with plasmid-encoded Cas proteins. Compositions and methods for a chromosomal integrated type I-F CRISPR-Cas system for programmable genome editing and robust gene regulation are provided. In some embodiments, the compositions and methods are effective to selectively and specifically edit and/or regulate the genome of multiple microbial species with diverse genotypes. Compositions and methods for gene editing and/or gene regulation in Pseudomonas spp, such as multiple strains of P. aeruginosa, are described.

The present invention relates to a promoter system inducing expression of 3-hydroxypropionic acid (3-HP) and a method of biologically producing 3-HP using the same. To improve production of 3-HP in a biological manner, continuous synthesis of new enzymes having enzyme activity is necessary. As a result of screening 3-HP reactive transcription regulators and 3-HP reactive promoters from several microorganisms including Pseudomonas denitrificans, it was confirmed that the transcriptions regulations and promoters are composed of LysR proteins and particular gene nucleotide sequences binding to the LysR proteins. Therefore, the 3-HP inducible system is expected to be effectively used to regulate 3-HP metabolic pathways.

The present invention relates to a promoter system inducing expression of 3-hydroxypropionic acid (3-HP) and a method of biologically producing 3-HP using the same. To improve production of 3-HP in a biological manner, continuous synthesis of new enzymes having enzyme activity is necessary. As a result of screening 3-HP reactive transcription regulators and 3-HP reactive promoters from several microorganisms including Pseudomonas denitrificans, it was confirmed that the transcriptions regulations and promoters are composed of LysR proteins and particular gene nucleotide sequences binding to the LysR proteins. Therefore, the 3-HP inducible system is expected to be effectively used to regulate 3-HP metabolic pathways.