In accordance with the present invention, CHO cells expressing a recombinant polypeptide of interest are grown in media where the amino acids, vitamins, phosphate, lipids and/or antioxidant optimization is utilized to manipulate and/or control the protein quality attributes of the polypeptides. Polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical compositions.

Disclosed herein is a nanovesicles from adult stem cells containing iron nanoparticles and its use. The nanovesicle according to the present disclosure provides effects of maximizing the efficacy of treating mesenchymal stem cells by pretreating cells with iron nanoparticles; reconstituting the cells in a nano-sized form to facilitate intravenous injection; and increasing the efficiency of targeting disease areas through magnet induction. In particular, the present disclosure can replace mesenchymal stem cells as a cell therapeutic agent, and it can be applied to various diseases as a novel biopharmaceutical drug because it can increase the function and efficiency of an exosome-based therapeutic agent.

The present invention relates to a method for providing a fermentation medium for the cultivation of at least one methanotrophic organism, or for the cultivation of a combination of organisms comprising at least one methanotrophic organism, the method comprises the step of: (i) providing a main growth solution; (ii) optionally sterilizing the main growth solution provided in step (i); (iii) providing a trace metal solution; and (iv) mixing the main growth solution (as provided in step (i) or (ii)) with the trace metal solution provided in step (iii) and providing the fermentation medium for the cultivation of at least one methanotrophic organism, or for the cultivation of a combination of organisms comprising at least one methanotrophic organism.

Various embodiments are described herein for creating a 3D human heart model for modelling arrythmias, wherein the method comprises seeding a structure with a mixture of human cardiomyocytes, cardiac fibroblasts and a fibrin mixture to form cardiac tissue; applying a plating media for settlement and compaction of the cardiac tissue; and adding an arrhythmogenic media to the cardiac tissue, where the arrhythmogenic media comprises methyl-beta cyclodextrin for disrupting calcium signaling.

The present invention provides a process for controlling the production of ethanol by microbial fermentation of gaseous substrates. More specifically, a process is provided for controlling ethanol productivity through addition of vitamins and a low cell retention time. In accordance with the process, vitamins B1, B5 and B7 are added in amounts that increase specific ethanol productivity. Cell retention times are maintained at low levels.

Methods and composition for production of T cells are provided. Also provided are therapeutic methods using engineered T cells. For example, in certain aspects methods include preparing three dimensional cell culture compositions comprising stroma cells and hematopoietic stem or progenitor cells in a serum-free medium for producing T cells.

The present disclosure provides a method for producing red blood cells by using peripheral blood as well as the produced red blood cells.

The present invention provides a pluripotent stem cell comprising a co-expression vector in which Runx1 and Hoxa9 are of in tandem, and a T cell differentiated therefrom and application thereof. In the present invention, Pluripotent stem cells inducibly co-expressing exogenous Runx1 and Hoxa9 are successfully established by introducing an exogenous vector co-expressing Runx1 and Hoxa9 into pluripotent stem cells. The pluripotent stem cells are directionally differentiated into T-lineage progenitor cells and will be developed into T cells. The pluripotent stem cell-derived T cells obtained by the method of the present invention are not only functionally normal but also have no tumorigenic risk.

Provided herein is a cultured meat product comprising a confluent serum-free insect muscle cell culture seeded on a food safe substrate. Further provided herein is a method for producing a cultured meat product comprising the steps of: culturing insect muscle cells on a food safe substrate in serum-free culture medium for a time sufficient for the cells to reach confluence. Also provided herein is a bioactuator comprising confluent insect muscle cells cultured in a flexible substrate to form muscle fibers.

Provided herein are nutrient media formulations and engineered growth factors, and methods thereof, useful for the production of slaughter-free meat.