This invention also relates to recombinant vectors expressing one or more of the human CMV (HCMV) glycoproteins US2, US3, US6 and US11 or corresponding functional rhesus CMV (RhCMV) homologues Rh182, Rh184, Rh185 or Rh189, methods of making them, uses for them, expression products from them, and uses for the expression products. This invention also relates to recombinant cytomegalovirus vectors vectors lacking one or more of the glycoproteins, methods of making them, uses for them, expression products from them, and uses for the expression products.

This invention also relates to recombinant vectors expressing one or more of the human CMV (HCMV) glycoproteins US2, US3, US6 and US11 or corresponding functional rhesus CMV (RhCMV) homologues Rh182, Rh184, Rh185 or Rh189, methods of making them, uses for them, expression products from them, and uses for the expression products. This invention also relates to recombinant cytomegalovirus vectors vectors lacking one or more of the glycoproteins, methods of making them, uses for them, expression products from them, and uses for the expression products.

This invention also relates to recombinant vectors expressing one or more of the human CMV (HCMV) glycoproteins US2, US3, US6 and US11 or corresponding functional rhesus CMV (RhCMV) homologues Rh182, Rh184, Rh185 or Rh189, methods of making them, uses for them, expression products from them, and uses for the expression products. This invention also relates to recombinant cytomegalovirus vectors vectors lacking one or more of the glycoproteins, methods of making them, uses for them, expression products from them, and uses for the expression products.

Provided are a human-induced pluripotent stem cell overexpressing TLX and use thereof. By enabling a human-induced pluripotent stem cell (hiPSC) to overexpress TLX or a truncation thereof, the self-driven differentiation of hiPSC into NSC is achieved. By employing this solution, not only the differentiation of hiPSC into NSC is accelerated, but also long-term stable in-vitro passaging of NSC is achieved, so that donor cells are provided for the treatment of nervous system diseases, cell therapy, and the like, and the obtained NSC exosome has good biological activity.

A tool virus derived from genetically engineered MCMV K181 strain for tracing MCMV infection, wherein the tool virus is a recombinant MCMV K181 strain comprising a tracing elements expression cassette and a BAC backbone, wherein the BAC backbone and the tracing elements expression cassette are inserted together between M06 open reading frame and M07 open reading frame of K181 strain genome; wherein the tracing elements expression cassette comprises three tracing elements coding sequences; and wherein the tracing elements expression cassette from 5 to 3 sequentially comprises a first fluorescent protein, a first linker, a second fluorescent protein, a second linker, a luciferase and a polyA.