The mutant strain of the alga Nannochloropsis is provided. A method for producing the mutant strain of the alga Nannochloropsis is also provided. Use of compounds produced from the mutant strain of the alga Nannochloropsis is further provided.

The mutant strain of the alga Nannochloropsis is provided. A method for producing the mutant strain of the alga Nannochloropsis is also provided. Use of compounds produced from the mutant strain of the alga Nannochloropsis is further provided.

The present invention relates to recombinant vectors, modified microorganisms, and methods for omega-3 polyunsaturated fatty acid production.

The present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene fragment 1 to gene fragment 6, and the nucleotide sequences are sequentially as shown in SEQ ID NO: 3 to SEQ ID NO: 8 in the Sequence Listing. The experiments prove that fermentation broth containing DHA and EPA can be obtained by fermenting Schizoochytrium limacinum HS01; the recombinant strain is obtained by introducing gene fragment 1 to gene fragment 6 into Schizoochytrium limacinum MYA-1381; the ability the recombinant strain for producing DHA and EPA is greatly improved. The bacteria provided by the invention, the six gene fragments, the protein encoded by these six gene fragments, the vector, the cell or the organism containing these six gene fragments all have important application values.

A method of producing lipids, containing the steps of: culturing a transformant into which a gene encoding at least one of the proteins selected from the group consisting of the following proteins (A) to (C) is introduced; and producing fatty acids or lipids containing the same as components:
(A) A protein consisting of the amino acid sequence of the 23.sup.rd to 146.sup.th amino acids set forth in SEQ ID NO: 1;
(B) A protein consisting of an amino acid sequence having 70% or more identity with the amino acid sequence of the protein (A), and having acyl carrier protein activity; and
(C) A protein containing the amino acid sequence of the protein (A) or (B), and having acyl carrier protein activity.

Disclosed herein are processes for reducing emulsion during the process of obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by maintaining the level of carbohydrate at less than 15 g/Kg in the fermentation broth. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

The present invention relates to an enzyme for producing hydroxy derivatives of polyunsaturated fatty acids, a method for producing hydroxy derivatives of polyunsaturated fatty acids using the same, and an SPMs complex composition containing the hydroxy derivatives of polyunsaturated fatty acids.

The enzyme may produce hydroxy derivatives of polyunsaturated fatty acids in a single reaction, and thus may be very usefully used for in vitro production. In addition, the SPMs complex composition containing the hydroxy derivatives of polyunsaturated fatty acids promotes collagen production, inhibits the production of NO and expression of TNF- and IL-6, increases the expression of filaggrin and loricrin, and thus may be usefully used for preventing or alleviating skin aging or wrinkles, strengthening skin barrier, or preventing, alleviating or treating skin diseases comprising atopic dermatitis.

The present invention relates to an enzyme for producing hydroxy derivatives of polyunsaturated fatty acids, a method for producing hydroxy derivatives of polyunsaturated fatty acids using the same, and an SPMs complex composition containing the hydroxy derivatives of polyunsaturated fatty acids.

The enzyme may produce hydroxy derivatives of polyunsaturated fatty acids in a single reaction, and thus may be very usefully used for in vitro production. In addition, the SPMs complex composition containing the hydroxy derivatives of polyunsaturated fatty acids promotes collagen production, inhibits the production of NO and expression of TNF- and IL-6, increases the expression of filaggrin and loricrin, and thus may be usefully used for preventing or alleviating skin aging or wrinkles, strengthening skin barrier, or preventing, alleviating or treating skin diseases comprising atopic dermatitis.

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.

The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.