To find a novel lipase suitable for processing of oils and fats and to use and apply the lipase. Provided are a novel lipase including an amino acid sequence having 90% or more identity with an amino acid sequence of SEQ ID NO: 1 or 2, and an enzyme preparation containing the same as an active ingredient. The enzyme preparation is used for modifying and improving the physical properties of oils and fats.

An enzymatic method for selective catalytic preparation of 4-octyl itaconate is provided, which belongs to the fields of biochemical engineering and enzymatic catalysis. The method uses a lipase or an enzyme preparation with a catalytic triplet structure (Ser-His-Asp or Ser-His-Gly) as a catalyst, itaconic acid and n-octanol or n-octanol-derived ester as raw materials to selectively catalyze the synthesis of 4-octyl itaconate in a solvent system or a solvent-free system. Compared with chemical synthesis routes of 4-octyl itaconate, the present method has the advantages of green, safety, and environmental protection. The obtained 4-octyl itaconate is one of the new small molecule compounds with anti-inflammatory and anti-viral properties.

The present invention relates to recombinant cells, particularly recombinant plant cells, which are capable of producing dihydrosterculic acid and/or derivatives thereof. The present invention also relates to methods of producing oil comprising dihydrosterculic acid and/or derivatives thereof.

An enzymatic method for preparing glyceryl butyrate, comprising: (1) carrying out an esterification reaction between n-butyric acid and glycerol using lipase as a catalyst, ethyl acetate and/or ethyl formate as an additive; (2) separating the reaction product, recovering the additive by distillation under reduced pressure to obtain glyceryl butyrate. The invention uses lipase as a catalyst to catalyze the esterification reaction of n-butyric acid and glycerin at normal temperature and normal pressure, reduces the energy consumption of the reaction. Also, the reaction condition is mild, no side reaction happens, and no water-carrying agent is used. In addition, the additive increases the catalytic efficiency of the lipase and the conversion rate of butyric acid.

An object of the present invention is to provide an enzyme showing random transesterification ability, which is suitable for food applications. Provided is a random transesterification lipase having high heat resistance in oils and fats.

A method of producing lipids, containing the steps of: culturing a transformant wherein the expressions of a gene encoding any one of the following proteins (A) to (F) and a gene encoding an acyl-ACP thioesterase are enhanced, and producing fatty acids or lipids containing these fatty acids as components:
(A) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 2;
(B) a protein consisting of an amino acid sequence having 89% or more identity with the amino acid sequence of the protein (A), and having acyl-CoA synthetase activity;
(C) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 4;
(D) a protein consisting of an amino acid sequence having 49% or more identity with the amino acid sequence of the protein (C), and having acyl-CoA synthetase activity;
(E) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 6; and
(F) a protein consisting of an amino acid sequence having 85% or more identity with the amino acid sequence of the protein (E), and having acyl-CoA synthetase activity.

The invention provides enzyme particles comprising an immobilized lipolytic enzyme, a siliceous material, an organic filter aid, and a water-soluble polyol selected from carbohydrates and sugar alcohols. The particles are suitable for enzymatic interesterification of triglycerides, and subsequent separation of the enzyme and triglycerides by filtration.

An immobilized enzyme Pickering emulsion reaction system and application thereof are provided, comprising immobilized enzymes with a mesoporous nanomaterial carrier, an oil phase and an aqueous phase for forming an emulsion, wherein the emulsion has a particle diameter of 10-80 m, which uses a reaction raw material as the oil phase, uses a butler solution as the aqueous phase, and uses the immobilized enzymes with the mesoporous nanomaterial carrier as both the catalyst and the emulsifier. Compared with conventional emulsions with additional organic reagents or emulsifiers, catalytic activity and stability the Pickering emulsion enzymatic reaction system of the present invention have been significantly improved. Products are easy to separate and purify, easy to reuse, and easy to scale up. The present invention has wider application scope, which is more conducive to environmental protection.

Methods to prepare vegetable oil compositions having an elevated ethyl ester content are provided.

The present invention addresses the problem of providing a method for effectively producing a target oil/fat that is rich in triglyceride, by using liberated by-products such as fatty acid ester originating from starting material oil/fat that is separated from the target oil/fat efficiently after the reaction when producing the target oil/fat that is rich in triglyceride by a transesterification reaction using, for example, oil/fat and fatty acid ester as starting materials.