The present invention relates to variant thioesterases and their use in plants, e.g., to increase enzymatic activity and to promote increased production of mid-chain length fatty acids (e.g., 8 to 14 carbons) and at desired ratios. Further disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods described herein.

Disclosed is a diacylglycerol acyltransferase 1, a recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1, and application thereof in production of triacylglycerol. The diacylglycerol acyltransferase 1 of the invention has a function of catalyzing synthesis of triacylglycerol. After the recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1 of the invention is subjected to induction culture for 48 h, the content of total fatty acid and triacylglycerol in the recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1 can be respectively increased by 1.94 folds and 12.09 folds as compared with those of Saccharomyces cerevisiae without the recombinant diacylglycerol acyltransferase 1. The instant invention provides a method for improving the ability of microorganisms to produce polyunsaturated fatty acids (PUFAs) by means of genetic engineering.

This disclosure provides methods for the chemical modification of triglycerides that are highly enriched in specific fatty acids and subsequent use thereof for producing functionally versatile polymers.

The invention relates to a mixture composition comprising glucolipids, to its use for producing formulations and to formulations comprising this mixture composition.

The present invention relates to the technical field of gene engineering and particularly relates to a method for constructing non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield, recombinant strain constructed by method, and application of recombinant strain. According to the present invention, a diacylglycerol acyltransferase gene (DGAT) is overexpressed in Mucor circinelloides WJ11 by a homologous recombination technology, and exogenous oil is added for fermentation, such that the non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield is constructed. Compared with the control strain Mc2075, the fat yield of the Mucor circinelloides is increased; and when the diacylglycerol acyltransferase (DGAT) is transformed into the uracil defective type of Mucor circinelloides WJ11, the fatty acid composition changes after fermentation, and the lipid content may reach 53% of dry cell weight after the fermentation condition is optimized.

The invention relates to the isolation of polyunsaturated fatty acids (PUFAs) from a lipids containing biomass. The main characteristics of the method are that a suspension of cells containing PUFAs is obtained and the cells are optionally lysed. The suspension is concentrated and then incubated for at least an hour at a controlled temperature while adding a specific range of base equivalents to biomass and maintaining a moderate pH. The isolation of lipid may be performed in the absence of organic solvents and without the addition of salt.

The invention relates to the isolation of polyunsaturated fatty acids (PUFAs) from a lipids containing biomass. The main characteristics of the method are that a suspension of cells containing PUFAs is obtained and the cells are optionally lysed. The suspension is concentrated and then incubated for at least an hour at a controlled temperature while adding a specific range of base equivalents to biomass and maintaining a moderate pH. The isolation of lipid may be performed in the absence of organic solvents and without the addition of salt.

An Aureobasidium pullulans recombinant strain with high-yield heavy oil and a construction method and application thereof are provided. The Aureobasidium pullulans recombinant strain is obtained by knocking out a pullulan synthetase PUL gene while overexpressing an ACL gene. The obtained Aureobasidium pullulans recombinant strain can significantly increase the yield of heavy oil. After 7-day fermentation with xylose as carbon source, the yield of the heavy oil of the recombinant strain reaches 19.4372 g/L, while the yield of the heavy oil of the original strain is 10.0325 g/L, i.e. the recombinant strain improves the yield by 93.74% compared with the original strain.

An Aureobasidium pullulans recombinant strain with high-yield heavy oil and a construction method and application thereof are provided. The Aureobasidium pullulans recombinant strain is obtained by knocking out a pullulan synthetase PUL gene while overexpressing an ACL gene. The obtained Aureobasidium pullulans recombinant strain can significantly increase the yield of heavy oil. After 7-day fermentation with xylose as carbon source, the yield of the heavy oil of the recombinant strain reaches 19.4372 g/L, while the yield of the heavy oil of the original strain is 10.0325 g/L, i.e. the recombinant strain improves the yield by 93.74% compared with the original strain.

Disclosed is oleaginous yeast and its application, belonging to the technical field of microorganisms. The oleaginous yeast provided by the disclosure has been deposited in China Center for Type Culture Collection on May 21, 2020, with an accession number of CCTCC NO: M 2020139. The oleaginous yeast provided by the disclosure can use a hydrolysate of cheap lignocellulose biomass as the substrate. Xylose and glucose in the hydrolysate are simultaneously utilized to produce oil, so the sugar utilization efficiency and production intensity are enhanced. After 8 days of fed-batch fermentation, the oil yield can reach 31.33 g/L, and the oil content can reach 60.83%. The waste resources can be reutilized, and the production cost is reduced, so the oleaginous yeast has wide application prospects.