The present invention relates to a cell capable of producing a fluorinated, a chlorinated or a brominated compound, methods for producing fluorinated, chlorinated or brominated compounds in a cell and expression systems therefor.

The disclosure generally relates to microorganisms, methods, and compositions for reducing methionine content of a diet of a subject or extending a lifespan of the subject and processes for preparing the microorganisms and compositions.

The disclosure generally relates to microorganisms, methods, and compositions for reducing methionine content of a diet of a subject or extending a lifespan of the subject and processes for preparing the microorganisms and compositions.

The present invention relates to an enzymatic synthesis of 4-ethynyl-2-deoxy nucleosides and analogs thereof, for example EFdA, that eliminates the use of protecting groups on the intermediates, improves the stereoselectivity of glycosylation and reduces the number of process steps needed to make said compounds. It also relates to the novel intermediates employed in the process.

The present invention relates to an enzymatic synthesis of 4-ethynyl-2-deoxy nucleosides and analogs thereof, for example EFdA, that eliminates the use of protecting groups on the intermediates, improves the stereoselectivity of glycosylation and reduces the number of process steps needed to make said compounds. It also relates to the novel intermediates employed in the process.

The present invention relates to a method for the high yield production of the anticancer nucleoside clofarabine, the method comprising the preparation of 2-chloroadenosine by enzymatic transglycosylation between 2-chloroadenine and nucleosides, benzoylation, isomerization, sulfonate ester formation, fluorination, and deprotection.

The present invention relates to a method for the high yield production of the anticancer nucleoside clofarabine, the method comprising the preparation of 2-chloroadenosine by enzymatic transglycosylation between 2-chloroadenine and nucleosides, benzoylation, isomerization, sulfonate ester formation, fluorination, and deprotection.

Metabolically-engineered yeast strains are provided. such as metabolically-engineered Saccharomyces cerevisiaestrains. producing high amounts of at least one. preferably all four natural cytokinins: trans-zeatin (tZ), trans-zeatin riboside (tZR). isopentenyladenine (iP) and isopenteny ladenine riboside (iPR).

The present invention relates to the field of genetic engineering and process engineering, and discloses an engineering strain producing cordycepin and its derivative 3-deoxyinosine, its preparation method and application. The method includes: codon optimizing genes Cm1 and Cm2; gene amplification and fragment recovering; methanol-induced promoter and terminator fragments; and inserting the genes into an expression vector and then transferring to Pichia pastoris for screening to obtain the engineering strain of Pichia pastoris of the present invention. The engineering strain can be applied to production of cordycepin and its derivative. The engineering strain prepared by the preparation method of the present invention has great advantages in producing cordycepin and its derivative. Due to the use of unique strains of Cordyceps militaris which have unique Cm1 and Cm2 genes, the efficiency of cordycepin production is greatly improved, and subsequently the green process of producing cordycepin and its derivative 3-deoxyinosine with carbon dioxide can be realized.

The present invention relates to the field of genetic engineering and process engineering, and discloses an engineering strain producing cordycepin and its derivative 3-deoxyinosine, its preparation method and application. The method includes: codon optimizing genes Cm1 and Cm2; gene amplification and fragment recovering; methanol-induced promoter and terminator fragments; and inserting the genes into an expression vector and then transferring to Pichia pastoris for screening to obtain the engineering strain of Pichia pastoris of the present invention. The engineering strain can be applied to production of cordycepin and its derivative. The engineering strain prepared by the preparation method of the present invention has great advantages in producing cordycepin and its derivative. Due to the use of unique strains of Cordyceps militaris which have unique Cm1 and Cm2 genes, the efficiency of cordycepin production is greatly improved, and subsequently the green process of producing cordycepin and its derivative 3-deoxyinosine with carbon dioxide can be realized.