Methods, apparatuses, and systems for analyzing microorganism strains in complex heterogeneous communities, determining functional relationships and interactions thereof, and diagnostics and biostate management and biostate temporal forecasting based thereon are disclosed. Methods for diagnostics, analytics, and treatments of states and state aberrations/deviations, including treatments, such as bioreactive modificators, such as bioreactive modificators comprising synthetic microbial ensembles, are also disclosed.

Methods, apparatuses, and systems for analyzing microorganism strains in complex heterogeneous communities, determining functional relationships and interactions thereof, and diagnostics and biostate management and biostate temporal forecasting based thereon are disclosed. Methods for diagnostics, analytics, and treatments of states and state aberrations/deviations, including treatments, such as bioreactive modificators, such as bioreactive modificators comprising synthetic microbial ensembles, are also disclosed.

The use of metabolic probes is well-established for determining cell viability and assessing drug cytotoxicity. Resazurin-based formulations, in particular, have found utility for determining susceptibilities of microorganisms to antimicrobials, specifically through their use in antibiotic susceptibility testing (AST). There is a strong need currently to shorten AST durations, thus resazurin formulations that produce signals earlier are advantageous. This need results from the slow state of clinical microbiology testing, which may leave patients exposed to unnecessary or ineffective broad-spectrum agents for prolonged periods of time. Another slow microbiology test is Gram staining, still most often performed manually in sequential steps. Speeding Gram typing, preferably with an automated platform, would also speed time-to-results and decrease manual workloads on medical technologists in clinical microbiology laboratories.

The use of metabolic probes is well-established for determining cell viability and assessing drug cytotoxicity. Resazurin-based formulations, in particular, have found utility for determining susceptibilities of microorganisms to antimicrobials, specifically through their use in antibiotic susceptibility testing (AST). There is a strong need currently to shorten AST durations, thus resazurin formulations that produce signals earlier are advantageous. This need results from the slow state of clinical microbiology testing, which may leave patients exposed to unnecessary or ineffective broad-spectrum agents for prolonged periods of time. Another slow microbiology test is Gram staining, still most often performed manually in sequential steps. Speeding Gram typing, preferably with an automated platform, would also speed time-to-results and decrease manual workloads on medical technologists in clinical microbiology laboratories.

Disclosed herein are methods determining susceptibility of bacteria in a sample from a subject suspected of having an infection to a plurality of antibiotics simultaneously, wherein the sample is tested without first isolating the bacteria from the sample.

Disclosed herein are methods determining susceptibility of bacteria in a sample from a subject suspected of having an infection to a plurality of antibiotics simultaneously, wherein the sample is tested without first isolating the bacteria from the sample.

An imaging method for earliest microbial growth detection. The method uses images to determined colony biomass, and the colony biomass determines when the colony can be picked for analysis for identification or antibiotic susceptibility testing. If the sample source is not a pure sample source additional incubation may be required to permit an increase in biomass of the colonies prior to pick.

An imaging method for earliest microbial growth detection. The method uses images to determined colony biomass, and the colony biomass determines when the colony can be picked for analysis for identification or antibiotic susceptibility testing. If the sample source is not a pure sample source additional incubation may be required to permit an increase in biomass of the colonies prior to pick.

Disclosed herein are methods determining susceptibility of bacteria in a sample from a subject suspected of having an infection to a plurality of antibiotics simultaneously, wherein the sample is tested without first isolating the bacteria from the sample.

Disclosed herein are methods determining susceptibility of bacteria in a sample from a subject suspected of having an infection to a plurality of antibiotics simultaneously, wherein the sample is tested without first isolating the bacteria from the sample.