A portable detector is disclosed for detecting certain analytes of interest, such as genetic material (e.g., nucleic acids). The detector includes a reading component for the detection of the analytes, and control circuitry for controlling operation of the reading component. Processing circuitry may be included to perform both primary analysis of acquired data, and where desired, secondary analysis. Where desired, some or all of the computationally intensive tasks may be off-loaded to enhance the portability and speed of the device. The device may incorporate various types of interface, technologies for reading and analysis, positioning system interfaces, and so forth. A number of exemplary use cases and methods are also disclosed.

Disclosed herein are embodiments of nanohoop compounds and conjugates thereof that can be used myriad biological applications. The nanohoop compounds described herein can exhibit beneficial properties that are useful in biotechnology, such as a fluorescent tag, probe, or label.

Disclosed herein are embodiments of nanohoop compounds and conjugates thereof that can be used myriad biological applications. The nanohoop compounds described herein can exhibit beneficial properties that are useful in biotechnology, such as a fluorescent tag, probe, or label.

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample.

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample.

Disclosed herein are methods, compositions, and kits related to the identification and/or quantification of target molecules.

Methods of detecting methylated CpG are provided. Accordingly, there is provided a method of determining CpG methylation status in a DNA sample, the method comprising: (a) subjecting the DNA sample to bisulfite conversion; (b) amplifying said DNA sample following said (a) to obtain an amplified DNA sample; (c) labeling CpG sites in said amplified DNA sample with a label to obtain a labeled DNA sample; (d) contacting said labeled DNA sample on an array comprising a plurality of probes for said DNA under conditions which allow specific hybridization between said plurality of probes and said DNA; and (e)detecting said hybridization, wherein an amount of said label is indicative of the CpG methylation status in said DNA sample.

Methods of detecting methylated CpG are provided. Accordingly, there is provided a method of determining CpG methylation status in a DNA sample, the method comprising: (a) subjecting the DNA sample to bisulfite conversion; (b) amplifying said DNA sample following said (a) to obtain an amplified DNA sample; (c) labeling CpG sites in said amplified DNA sample with a label to obtain a labeled DNA sample; (d) contacting said labeled DNA sample on an array comprising a plurality of probes for said DNA under conditions which allow specific hybridization between said plurality of probes and said DNA; and (e)detecting said hybridization, wherein an amount of said label is indicative of the CpG methylation status in said DNA sample.

The invention includes, at minimum, an integrated, automated system for diagnosis of antibiotic resistance genes that performs microarray hybridization, washes, and reading, using a plurality of high-density glass and/or polymer microarray chips. The system may include one or more ring or circle-shaped microarray chips formed from glass and/or a polymer. The system may also include well plates pre-treated with one or more of fluorescently labeled primers, polymerase, dNTPs or other chemicals or reagents used to multiplex PCR identification of antibiotic resistance genes. The system may also include one or more of 96 or 128 sized well plates. The system may also include one or more centrifugation tubes having a nanopore filter fixed in place and/or a viscous solution for isolating smaller pathogens from larger host eukaryotic cells.

The invention includes, at minimum, an integrated, automated system for diagnosis of antibiotic resistance genes that performs microarray hybridization, washes, and reading, using a plurality of high-density glass and/or polymer microarray chips. The system may include one or more ring or circle-shaped microarray chips formed from glass and/or a polymer. The system may also include well plates pre-treated with one or more of fluorescently labeled primers, polymerase, dNTPs or other chemicals or reagents used to multiplex PCR identification of antibiotic resistance genes. The system may also include one or more of 96 or 128 sized well plates. The system may also include one or more centrifugation tubes having a nanopore filter fixed in place and/or a viscous solution for isolating smaller pathogens from larger host eukaryotic cells.