The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

This invention provides a simple analysis method by a PCR method that simultaneously performs, in a single container, separating nucleic acid of pathogens contained in a tissue fragment and preparing a PCR buffer solution, and a kit used for the analysis method. A method for detecting a pathogen includes: (1) obtaining a liquid specimen mixture by adding a tissue fragment containing a pathogen to a PCR buffer solution containing a proteolytic enzyme; (2) heating the liquid specimen mixture at a first temperature; (3) further heating at a second temperature; (4) performing PCR by adding a portion of the liquid mixture obtained in (3) above to a solid composition for PCR reaction containing DNA polymerase and one or more kinds of PCR primer pair; and (5) detecting a PCR product generated in (4) above.

This invention provides a simple analysis method by a PCR method that simultaneously performs, in a single container, separating nucleic acid of pathogens contained in a tissue fragment and preparing a PCR buffer solution, and a kit used for the analysis method. A method for detecting a pathogen includes: (1) obtaining a liquid specimen mixture by adding a tissue fragment containing a pathogen to a PCR buffer solution containing a proteolytic enzyme; (2) heating the liquid specimen mixture at a first temperature; (3) further heating at a second temperature; (4) performing PCR by adding a portion of the liquid mixture obtained in (3) above to a solid composition for PCR reaction containing DNA polymerase and one or more kinds of PCR primer pair; and (5) detecting a PCR product generated in (4) above.

The present invention relates to the use of next generation technologies coupled with viability and pathogenicity profiles to determine the threat of microbes in the environment. The invention relates to methods for identifying a pathogenicity and viability profile of microbes from collected samples.

The present invention relates to the use of next generation technologies coupled with viability and pathogenicity profiles to determine the threat of microbes in the environment. The invention relates to methods for identifying a pathogenicity and viability profile of microbes from collected samples.

Described are methods for isolating, amplifying and analyzing nucleic acids from live cells in mixed cultures suspected of containing living cells, dead cells, and/or free nucleic acids released extracellularly from dead or dying cells. Also provided are processed test samples and kits for preparation thereof.

Described are methods for isolating, amplifying and analyzing nucleic acids from live cells in mixed cultures suspected of containing living cells, dead cells, and/or free nucleic acids released extracellularly from dead or dying cells. Also provided are processed test samples and kits for preparation thereof.

Compositions and methods for detecting Trichomonas vaginalis are provided.

Compositions and methods for detecting Trichomonas vaginalis are provided.