Hydrogel-based scaffolds useful for promoting pulp cell growth and biosynthesis, regulating pulp cell migration and morphology, or both as well as methods for their production and use are provided.

The present disclosure relates to a method for producing dental pulp-derived cells enriched with pluripotent stem cells including: (a) digesting dental pulp with a protease to prepare a dental pulp suspension; (b) culturing the suspension to proliferate pluripotent stem cells contained in the suspension; (c) freezing the proliferated pluripotent stem cells in a state in which the pluripotent stem cells are suspended in a first cryopreservation liquid; (d) thawing the frozen pluripotent stem cells; (e) culturing the thawed pluripotent stem cells in a state in which the pluripotent stem cells are adhered to surfaces of particles to proliferate the pluripotent stem cells on the surfaces of the particles; and (f) bringing the particles into contact with a protease to separate the pluripotent stem cells adhered to the surfaces of the particles from the particles.

This invention relates to exosome compositions and methods of using them.

The present disclosure provides methods of improving cerebral function or preventing loss of cerebral function and/or treating or preventing a movement disorder and/or regenerating cerebral neurons in a subject who has suffered a stroke, the method comprising administering to the subject a population of cells enriched for STRO-1.sup.+ cells and/or progeny thereof and/or soluble factors derived therefrom.

The disclosure relates to dental pulp stem cells, method of preparing dental pulp stem cells, and methods of treating a patient with nervous tissue injuries by using dental pulp stem cells.

The present invention relates to the field of adult stem cells of embryonic neural crest origin, in particular, multifunctional immature dental pulp stem cells (IDPSCs). The invention discloses useful compositions and methods for the prevention or treatment of a wide range of diseases that lead to or derived from bone marrow failure (BMF), Acute Radiation Syndromes, or chemical injury through the use of isolated IDPSC population with minimal in vitro manipulation.

Provided is a method for producing an economical bovine serum composition containing many factors useful for cell proliferation. The method includes a step of performing an anticoagulation treatment of bovine whole blood with an anticoagulant, a step of obtaining a buffy coat and a fraction with a heavier specific gravity than that of the buffy coat from the anticoagulated whole blood, and a step of promoting and activating an interaction between the obtained leukocytes and platelets at a given temperature for not less than a given time to cause secretion or release of a humoral factor from the leukocytes and/or platelets and performing a recoagulation treatment of blood components including the humoral factor with a re-coagulating agent.

PROCESS OF CRYOPRESERVATION OF STEM CELLS OBTAINED FROM THE PULP OF DECIDUOUS TEETH is a process of collection of stem cells from deciduous teeth for freezing and storing, comprises the sequence of procedures from the collection of the deciduous teeth to the storage of stem cells in favourable conditions to its application when and if necessary, and for the operationalization of the process a Collection Kit was developed, in order to allow the Dentist to perform the collection in its own practice according to pre-set technical protocols and timing for sending, which was called the R-Crio Collection Kit; finally the opening of a further possibility of protocol from collecting until the storage and subsequent thawing of the stem cells form the deciduous pulp for application in therapies and studies involving Regenerative Biomedicine.

The present invention relates to an aqueous extract useful notably for the regeneration of human or animal cells, notably mammalian cells, comprising an aqueous extract free of any insoluble solid debris of ground planarian organisms having a regeneration capacity, containing at least the intracellular components of the cells of said organisms of a cell lysate of said cells of said organisms.

The purpose of the present invention is to provide immortalized stem cells for producing culture supernatant that can be used in the treatment of human diseases. Provided is a method for producing immortalized stem cells, the method being provided with: a step for producing a DNA fragment that includes a telomerase reverse transcriptase and at least one gene selected from the group consisting of Bmi-1, human papilloma virus E6, and human papilloma virus E7; a step for producing a viral vector that incorporates the DNA fragment including the gene; a step for transfecting the viral vector into mammalian stem cells and introducing the gene into the stem cells; and a step for culturing the stem cells into which the gene was introduced and using a drug to select immortalized stem cells.