A process for purifying a composition comprising water, a target substance, impurities and optionally cells, the process comprising the steps (A) and (B): (A) preparing a liquid feedstock by performing step (Ai) and/or (Aii) on the composition: (Ai) removing at least some of the cells from the composition; (Aii) concentrating the composition by removing water therefrom; and (B) passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified target substance and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v). The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

The present invention relates to a method for purification of viral vectors, more closely it relates to purification of viral vectors from producer cells by using a single automated process. The method comprises the following steps: a) adding producer cells and cell lysis buffer to a processing container; b) mixing said producer cells and cell lysis buffer in said processing container to obtain a mixture; c) flowing said mixture through a chromatography column for purification of viral vectors, wherein the viral vectors are adsorbed on said chromatography column; and d) eluting viral vectors from the chromatography column into a product container.

The present invention relates to a method for separating a sample comprising a nucleic acid, the method comprising: (a) loading a sample comprising a nucleic acid onto an anion-exchange column; and (b) eluting the sample from the anion-exchange column using a salt gradient, wherein a mobile phase used in the eluting step comprises a mild ion-pairing cation, and wherein a column temperature of the anion-exchange column is greater than 30° C. The present invention also relates to a method for determining one or more calorimetric properties of a nucleic acid using anion-exchange chromatography.

Apparatus for purifying a liquid comprising a target substance comprising at least two units arranged in series such that the feed stream of the second and any subsequent units comprises the product stream from a downstream unit, wherein each unit comprises specified components (i) to (vi), including a a switchable bypass assembly. Also claimed are the units and a flowpath assembly. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

The present disclosure generally relates to devices and methods for effecting epitachophoresis. Epitachophoresis may be used to effect sample analysis, such as by selective separation, detection, extraction, and/or pre-concentration of target analytes such as, for example, DNA, RNA, and/or other biological molecules. Said target analytes may be collected following epitachophoresis and used for desired downstream applications and further analysis.

Described herein is a bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput nucleic acid extraction and purification. BCFFF uses a membrane ionic transistor to sustain low-ionic strength in a localized region at a junction, such that the resulting high field can selectively isolate high-charge density nucleic acids from the main flow channel and insert them into a standardized buffer in a side channel that bifurcates from the junction. The BCFFF platform can be used for isolation of both long dsDNAs and short miRNAs, without changing the device configuration or the operation protocol. BCFFF results in high-efficiency (>85%) concentration-independent DNA extraction and 40% net qRT-PCR miRNA yield from plasma, which is significantly higher than any other commercial liquid and solid extraction technologies.

A method of single strand RNA purification employing an anion exchanger comprising the steps applying a sample containing single-stranded RNA to an anion exchanger, washing the anion exchanger at a first temperature in the range of 18° C. to 25° C. with a first salt solution having a first ionic strength in the range 0.5 M to 12.0 M, eluting the single stranded RNA by a second salt solution having a second ionic strength at a second temperature in the range of 35° C. to 80° C.,
with the proviso that the first ionic strength is at least 0.5 M higher than the second ionic strength.

A method of single-stranded RNA purification comprising the steps: applying a sample containing single-stranded RNA to a solid phase dominantly or exclusively bearing both primary and secondary amino groups on its surface under conditions sufficient to bind at least predominantly the single-stranded RNA, eluting the single-stranded RNA adsorbed on the surface of the solid phase from the solid surface by means of an elution buffer having a higher concentration of comprising soluble pyrophosphate anions than needed for desorbing double-stranded RNA.

Provided herein are methods, reagents, and kits for the isolation of sperm cells from other cell types and the purification of sperm-associated DNA from a complex sample. In particular, methods, reagents, and kits are provided for the separation of intact sperm cells from epithelial cells via binding of sperm cells to a cation exchange resin, and the extraction of sperm-associated DNA therefrom.

A CE based method and kit for the determination of the size and purity of an AAV genome which relies on Capillary Electrophoresis-Laser Induced Fluorescence (CE-LIF) analysis. These methods and kits are capable of detecting intact and partial genomes in a virus vectors such as adeno-associated viruses as well as remove small size impurities. In one example, the method can include creating a nucleic acid ladder using CE-LIF, releasing the genome from within an adeno-associated virus, purifying said genome and analyzing said genome using CE-LIF and comparing the results of the analysis of the genome to the nucleic acid ladder to determine a size of nucleic acids in the genome.