A process for producing cellulolytic and/or hemicellulolytic enzymes with a strain of microorganism belonging to the family of filamentous fungi. The process includes growing the fungi in an aqueous culture medium, in the presence of at least one carbon-based growth substrate, in a stirred and aerated bioreactor. It also includes the production of enzymes, starting with the aqueous culture medium in the presence of at least one inductive carbon-based substrate and also inducing the production of hydrophobins. Further, at least a portion of the hydrophobins produced in step (b) are reintroduced into the growth step (a).

An engineered yeast strain capable of efficient fermentation of xylose to ethanol, and methods of making and using the strain, are provided

An engineered yeast strain capable of efficient fermentation of xylose to ethanol, and methods of making and using the strain, are provided

The present disclosure generally relates to a method for treatment of a lignocellulosic biomass. The method comprises the steps of pretreating the lignocellulosic biomass material in a pretreatment arrangement to form a pretreated biomass slurry; optionally hydrolysing the pretreated biomass slurry in a hydrolysis unit; retrieving gaseous SO2 from the pretreatment arrangement to provide an SO2 gas stream; treating the SO2 gas stream with an alkaline solution to provide a sulfite/bisulfite solution; supplying at least a part of the sulfite/bisulfite solution to at least one of: a) the lignocellulosic biomass material prior to pretreating the lignocellulosic biomass material in the pretreatment arrangement; b) the lignocellulosic biomass material n the pretreatment arrangement; c) the pretreated biomass slurry discharged from the reactor vessel; d) the hydrolysate or fermentable sugars of the hydrolysate.

Embodiments provide a modified microbe capable of growing in or fermenting a solution, or lignocellulosic hydrolysate, comprising ferulic acid and/or coniferyl aldehyde. The microbe has one or more modifications to provide: (a) a decrease in copy number or expression of a BNA7 gene; (b) an increase in copy number or expression of one or more pentose phosphate pathway genes; and/or (c) localization of one or more products of the pentose phosphate pathway genes to the mitochondria or endoplasmic reticulum. Also provided is a microbe having modified expression or copy number of BNA7 and/or one or more of the pentose phosphate pathway genes. The pentose phosphate pathway genes may in certain embodiments be selected from at least one of ZWF1, TKL1, RPE1 and GND1. Also provided is a method for fermenting a substrate comprising ferulic acid and/or coniferyl aldehyde to produce a fermentation product.

This disclosure describes processes for using a single cellulosic feedstock or a combination of two or more different cellulosic feedstocks with a starch component to produce a fermented product. The process includes separating the components of the cellulosic feedstocks with fractionation, pretreating a component with wet fractionation with chemicals, hydrolysis and fermenta-tion of the pretreated feedstock(s) to produce cellulosic biofuel. The process may include combining the cellulosic feedstock(s) with other components to a cook and/or a fermentation process, distilling and dehydrating the combined components to produce the biofuel. The process may also include producing a whole stillage stream from the feedstock(s) and mechanically processing the whole stillage stream to produce a high-value protein animal feed.

This disclosure describes processes for using a single cellulosic feedstock or a combination of two or more different cellulosic feedstocks with a starch component to produce a fermented product. The process includes separating the components of the cellulosic feedstocks with fractionation, pretreating a component with wet fractionation with chemicals, hydrolysis and fermenta-tion of the pretreated feedstock(s) to produce cellulosic biofuel. The process may include combining the cellulosic feedstock(s) with other components to a cook and/or a fermentation process, distilling and dehydrating the combined components to produce the biofuel. The process may also include producing a whole stillage stream from the feedstock(s) and mechanically processing the whole stillage stream to produce a high-value protein animal feed.

The invention relates to a method and an apparatus for producing a soluble carbohydrate containing fraction (10), in which lignocellulose material (3) formed by treating plant based raw material (1) is conducted into a separation stage (4). The method comprises at least one solid-liquid separation stage (4) for separating a soluble carbohydrate containing fraction (10) and/or a washing filtrate (12) from lignocellulose material (3), and at least a part of the soluble carbohydrate containing fraction (10) and/or the washing filtrate (12) is recirculated to the lignocellulose material (3) for increasing concentration of the soluble carbohydrate containing fraction, and solids (11) and at least a part of the soluble carbohydrate containing fraction (10) are supplied out from the separation stage. Further, the invention relates to the soluble carbohydrate containing fraction and the solid fraction, and their uses.

The invention relates to a method and an apparatus for producing a soluble carbohydrate containing fraction (10), in which lignocellulose material (3) formed by treating plant based raw material (1) is conducted into a separation stage (4). The method comprises at least one solid-liquid separation stage (4) for separating a soluble carbohydrate containing fraction (10) and/or a washing filtrate (12) from lignocellulose material (3), and at least a part of the soluble carbohydrate containing fraction (10) and/or the washing filtrate (12) is recirculated to the lignocellulose material (3) for increasing concentration of the soluble carbohydrate containing fraction, and solids (11) and at least a part of the soluble carbohydrate containing fraction (10) are supplied out from the separation stage. Further, the invention relates to the soluble carbohydrate containing fraction and the solid fraction, and their uses.

A hemp biodegradable absorbent according to various aspects of the present technology comprises receiving hemp biomass and processing the biomass into a desired particle range through a separation process. The separation process may comprise an initial filtration to separate out particles having a size of greater than 500 μm, pulverizing or sifting the remaining particles into a plurality of grades in a range of 0.5 μm to 50 μm, and selecting at least one grade of particles to form the absorbent. The selected particles are dried to a moisture content of less than about 10% to create a hemp biodegradable absorbent base that can be used to form various types of absorbent products that may provide an absorbent capacity of between about at least about 300% and up to about 600% by weight. The hemp biodegradable absorbent may also be effective at absorbing both oil-based and water-based liquids.