A method of transforming a mitochondrion of a plant cell to express a nitrogenase enzyme includes exposing the plant cell to a mitochondrial-targeting nanocarrier polypeptide and one or more nucleic acids encoding the nitrogenase enzyme. The one or more genes encoding the nitrogenase enzyme can be one or more Klebsiella nif genes. The method can be used to generate plants which have the capability of fixing atmospheric elemental nitrogen.

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated of applied with a recombinant DNA construct comprising a sequence encoding one or more genome editing reagents for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

The present invention relates to a method of making bioluminescent plants by introducing luciferase genes and luciferin biosynthesis genes into plant chloroplasts.

Materials and methods for creating genome-engineered plants with non-transgenic methods are provided herein.

The present disclosure provides compositions and methods for delivering a polynucleotide from the exterior surface of a plant or plant part into the interior of a plant cell. More specifically, the present disclosure relates to compositions comprising at least one polynucleotide and at least one agent that is able to disrupt at least one barrier of the plant or plant part.

Systems and methods are provided for transfecting immune competent cells with RNA at high efficiency and viability.

Provided is a transgenic killer cell, the genome of which is stably integrated with a coding sequence comprising an antibody of a human Fc section, or an expression cassette of a coding sequence comprising a chimeric antigen receptor or an inhibitory or agonistic antibody, and an inverted terminal repeat sequence from a transposon at both ends. Also provided is a pharmaceutical composition comprising the transgenic killer cell, and uses thereof.

The present invention refers to the transfection of cells using a conjugate comprising at least one saccharide residue at least one nucleosidic component selected from nucleic acids, nucleosides and nucleotides. This conjugate is suitable for the transfection of prokaryotic and eukaryotic cells such as plant cells or mammalian cells including human cells with high efficacy. Thus, a new delivery vehicle for therapeutic molecules including antisense molecules, sRNA molecules, miRNA molecules, antagomirs or precursors of such molecules, as well as the therapeutic nucleosides or nucleotides, is provided. Further, a convenient strategy for developing new lines of plants that exhibit particular traits is provided.

The object of the present invention is to provide a method of introducing a nucleic acid into plant cells, which is simple and widely applicable to various types of plant cells and nucleic acids. The present invention relates to a method of introducing a nucleic acid into a target plant cell, comprising a step of forming a complex by bringing a carrier peptide, wherein the carrier peptide comprises a cell-penetrating sequence and a polycationic sequence, into contact with a nucleic acid and a step of bringing the obtained complex into contact with a target plant cell.

Systems and methods are provided for transfecting immune competent cells with RNA at high efficiency and viability.