Method and apparatus for feeding said molds laterally and/or between stations of differing heights. The method and apparatus of this invention utilize walking-beam-type conveyors having spaced apart fixed outboard rails and a central reciprocating rail. Walking-beam conveyors can be disposed in perpendicular directions with a junction therebetween that allows for a perpendicular change in direction. The method and apparatus of this invention include lift, leveling, and/or lateral transfer mechanisms for lateral placement of the stations relative to the conveyance path.

The present invention relates to a method for introducing a substance into a plant. The method of the present invention comprises the steps of: obtaining an enzymatically treated and isolated fertilized egg cell by (1-i) isolating a fertilized egg cell from a plant tissue containing a fertilized egg cell, and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, (1-ii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and then isolating the fertilized egg cell that has been enzymatically treated, (1-iii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and simultaneously isolating the fertilized egg cell that has been enzymatically treated, (1-iv) isolating an egg cell and a sperm cell from a plant to produce a fertilized egg by fusing the cells, and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, or (1-v) treating a plant tissue containing an egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and then isolating the egg cell that has been enzymatically treated, and further fusing the egg cell with an isolated sperm cell; and (2) introducing a substance selected from the group consisting of nucleic acids, proteins, and peptides into the resultant enzymatically treated and isolated fertilized egg cell.

A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.

Nano-sized delivery vehicles utilize carbon nanotubes (CNTs) for the generic, targeted and passive transport of biomolecules into plant cells.

An object of the present invention is to provide a plant transformation method that is convenient and is widely applicable to various types of plant cells and nucleic acids. The present invention relates to a method for transforming a target plant, comprising the steps of: a) contacting a carrier peptide comprising a cell-penetrating sequence and a polycation sequence with a nucleic acid to form a complex; b) contacting the obtained complex with a cell of a meristem of the target plant to transfer the nucleic acid to the genome; c) allowing the meristem to grow; and d) selecting a plant harboring the transferred nucleic acid.

The present invention relates to a method for introducing a substance into a plant. The method of the present invention comprises introducing a substance into a plant germ cell with incomplete cell wall formation.

Systems and methods are provided for transfecting immune competent cells with RNA at high efficiency and viability.

The invention relates to a process for delivering a substance, optionally a compound, vector or nanomaterial, to a plant. The process comprises providing a plant application medium comprising a substance, a carrier medium and micro- and/or nanobubbles of at least one gas; and applying the plant application medium to a locus of a plant. The substance enters at least one plant tissue of the plant. The substance may be one or more substances for inducing a change in a phenotype, chemistry or physiology of a plant, for example an epigenetic regulator. The present invention also relates to a system for delivering a substance to a plant and to media to be applied to a plant.

A soybean cultivar designated 77130123 is disclosed. The invention relates to the seeds of soybean cultivar 77130123, to the plants of soybean cultivar 77130123, to the plant parts of soybean cultivar 77130123, and to methods for producing progeny of soybean cultivar 77130123. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 77130123. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 77130123, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 77130123 with another soybean cultivar.

A soybean cultivar designated 63301112 is disclosed. The invention relates to the seeds of soybean cultivar 63301112, to the plants of soybean cultivar 63301112, to the plant parts of soybean cultivar 63301112, and to methods for producing progeny of soybean cultivar 63301112. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 63301112. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 63301112, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 63301112 with another soybean cultivar.