The present invention relates to methods for inducing apomixis in a plant, methods for the production of apomictic plants and the plants and plant seeds obtained thereby.

The present invention relates to the regulation of transgene expression in plants through a transactivation system which comprises the following: (1) a promoter comprising LexA binding sites; and (2) a fusion transactivator protein comprising a LexA DNA-binding domain and an activation domain, such as the transactivation domain of a C-repeat binding factor protein, for example, from a dehydration responsive element (DREB) factor.

The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.

This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.

The present invention provides methods of identifying and producing dominant negative MSH1 genes in plants and methods of using plants comprising dominant negative MSH1 genes for Msh1 suppression.

Plant genes regulated by transcription factors that control the gene network response to an environmental perturbation or signal are described. This class of genes responds to the perturbation of a transcription factor and the signal it transduces, but surprisingly, without stable binding of the transcription factor. These genes represent members of the dark matter of metabolic regulatory circuits. The invention involves the transgenic manipulation of these response genes and/or the genes encoding their regulatory transcription factors in plants so that their respective gene products are either overexpressed or underexpressed in the plant in order to confer a desired phenotype. The invention also relates to a rapid technique named TARGET (transient assay reporting genome-wide effects of transcription factors) for determining such response genes and their transcription factors by perturbation of the expression of the transcription factors of interest in protoplasts of any plant species.

Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.

The present disclosure provides synthetic repressor constructs and the proteins encoded therein, as well as synthetic repressible promoter constructs for use in combination with the synthetic repressor constructs/synthetic repressors disclosed herein. Various combinations of synthetic repressor constructs and synthetic repressible promoter constructs are also provided in synthetic genetic circuits for modifying expression of a protein of interest in a plant cell.

Nucleotide sequences encoding DREB2 polypeptides are provided herein, along with plants and cells having increased levels of DREB2 gene expression, increased levels of DREB2 transcription factor activity, or both. Plants with increased levels of at least one DREB2 gene expression that exhibit increased yield, increased abiotic stress tolerance, or any combination of these, are provided. Methods for increasing yield, and abiotic stress tolerance in plants, by modulating DREB2 gene expression or activity, are also provided.

The invention relates to plants that contain higher proportions of mannans. Such plants express transcription factors that increase the expression of CSLA9, a mannan synthase.